Biodegradation of Endosulfan as an Organochlorine Pesticide with Pseudomonas plecoglocissida Transfected by LinA Gene

Masoumeh Aslani Mehr 1 ,
Taghi Naserpour Farivar 1 , *
Reza Najafipour 1
Amir Peymani 2
Safar Ali Alizadeh 1
Pouran Johari 1

Authors Information

1 Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran
2 Medical Microbiology Research Center,Qazvin University of Medical Sciences, Qazvin, Iran
Article information
  • Biotechnology and Health Sciences: February 2018, 5 (1); e45306
  • Published Online: March 8, 2017
  • Article Type: Research Article
  • Received: January 2, 2017
  • Revised: January 12, 2017
  • Accepted: January 16, 2017
  • DOI: 10.5812/bhs.45306

To Cite: Aslani Mehr M, Naserpour Farivar T, Najafipour R, Peymani A, Alizadeh S A, et al. Biodegradation of Endosulfan as an Organochlorine Pesticide with Pseudomonas plecoglocissida Transfected by LinA Gene, Biotech Health Sci. 2018 ; 5(1):e45306. doi: 10.5812/bhs.45306.

Copyright © 2017, Qazvin University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited
1. Background
2. Objectives
3. Methods
4. Results and Discussion
  • 1. Mackie JC, Dharmarathne WNK, Kennedy EM, Stockenhuber M, editors. Non-Oxidative Thermal Decomposition of Endosulfan: II. Mechanism of Decomposition. Proceedings of the Australian Combustion Symposium. 2015.
  • 2. Kulkarni UK, Kulkarni KV, Pardeshi RK, Mane DV. High-performance thin-layer chromatographic detection of endosulfan from biological samples. J Planar Chrom. 2016;29(5):391-3. doi: 10.1556/1006.2016.29.5.11.
  • 3. Eddleston M, Karalliedde L, Buckley N, Fernando R, Hutchinson G, Isbister G, et al. Pesticide poisoning in the developing world–a minimum pesticides list. Lancet. 2002;360(9340):1163-7. [PubMed: 12387969].
  • 4. Sogorb MA, Vilanova E, Carrera V. Future applications of phosphotriesterases in the prophylaxis and treatment of organophosporus insecticide and nerve agent poisonings. Toxicol Lett. 2004;151(1):219-33. doi: 15177657].
  • 5. Mulbry WW, Karns JS. Parathion hydrolase specified by the Flavobacterium opd gene: relationship between the gene and protein. J Bacteriol. 1989;171(12):6740-6. [PubMed: 2556372].
  • 6. Dumas DP, Caldwell SR, Wild JR, Raushel FM. Purification and properties of the phosphotriesterase from Pseudomonas diminuta. J Biol Chem. 1989;264(33):19659-65. [PubMed: 2555328].
  • 7. Wolber PK. Bacterial ice nucleation. Adv Microb Physiol. 1993;34:203-37. [PubMed: 8452093].
  • 8. Geetha M, Fulekar MH. Bioremediation of pesticides in surface soil treatment unit using microbial consortia. Afr J Environ Sci Technol. 2008;2(2):36-45.
  • 9. Park SC, Shimamura I, Fukunaga M, Mori KI, Nakai T. Isolation of bacteriophages specific to a fish pathogen, Pseudomonas plecoglossicida, as a candidate for disease control. Appl Environ Microbiol. 2000;66(4):1416-22. [PubMed: 10742221].
  • 10. Fulekar MH, Geetha M. Bioremediation of Chlorpyrifos by Pseudomonas aeruginosa using scale up technique. J Appl Biosci. 2008;12:657-60.
  • 11. Cetın AK, Gur N, Fırat Z. Growth rate of Scenedesmus acutus in laboratory cultures exposed to diazinon. Afr J Biotechnol. 2011;10(34):6540-3.
  • 12. Iyer R, Stepanov VG, Iken B. Isolation and molecular characterization of a novel pseudomonas putida strain capable of degrading organophosphate and aromatic compounds. Adv Biol Chem. 2013;3(6):564-78. doi: 10.4236/abc.2013.36065.
  • 13. Shivaramaiah HM, Kennedy IR. Biodegradation of endosulfan by a soil bacterium. J Environ Sci Health B. 2006;41(6):895-905. doi: 16893778].
  • 14. Borji A, Naserpour Farivar G, Johari P, Naserpour Farivar T, Senemari S, Karimi G. Cleaning From the Inside: Biodegradation of Organophosphate Pesticides by Pseudomonas plecoglossicida. Biotechnol Health Sci. 2014;1(1). doi: 10.17795/bhs-19193.
  • 15. Casabar RCT. Metabolism of Endosulfan-  by Human Liver Microsomes and Its Utility as a Simultaneous in Vitro Probe for CYP2B6 and CYP3A4. Drug Metabol Disposit. 2006;34(10):1779-85. doi: 10.1124/dmd.106.010199.
  • 16. Nagata Y, Hatta T, Imai R, Kimbara K, Fukuda M, Yano K, et al. Purification and Characterization ofγ-Hexachlorocyclohexane (γ-HCH) Dehydrochlorinase (LinA) fromPseudomonas paucimobilis. Biosci Biotechnol Biochem. 2014;57(9):1582-3. doi: 10.1271/bbb.57.1582.
  • 17. Yang J, Liu R, Song W, Yang Y, Cui F, Qiao C. Construction of a genetically engineered microorganism that simultaneously degrades organochlorine and organophosphate pesticides. Appl Biochem Biotechnol. 2012;166(3):590-8. doi: 22139730].
  • 18. Boubakri H, Beuf M, Simonet P, Vogel TM. Development of metagenomic DNA shuffling for the construction of a xenobiotic gene. Gene. 2006;375:87-94. doi: 16690231].