Biodegradation of Paraoxan as an Organophosphate Pesticide with Pseudomonas plecoglocissida Transfected by opd Gene

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Biodegradation of Paraoxan as an Organophosphate Pesticide with Pseudomonas plecoglocissida Transfected by opd Gene

Taghi Naserpour Farivar 1 , *
Amir Peymani 2
Reza Najafipour 1
Masoumeh Aslani Mehr 1
Safar Ali Alizadeh 1
Pouran Johari 1

Authors Information

1 Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran
2 Medical Microbiology Research Center
Article information
  • Biotechnology and Health Sciences: February 2017, 4 (1); e13435
  • Published Online: February 12, 2017
  • Article Type: Research Article
  • Received: December 29, 2016
  • Revised: January 17, 2017
  • Accepted: January 17, 2017
  • DOI: 10.5812/bhs.13435

To Cite: Naserpour Farivar T, Peymani A, Najafipour R, Aslani Mehr M, Alizadeh S A, et al. Biodegradation of Paraoxan as an Organophosphate Pesticide with Pseudomonas plecoglocissida Transfected by opd Gene, Biotech Health Sci. 2017 ;4(1):e13435. doi: 10.5812/bhs.13435.

Copyright: Copyright © 2017, Biotechnology and Health Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results and Discussion
  • 1. Eddleston M, Karalliedde L, Buckley N, Fernando R, Hutchinson G, Isbister G, et al. Pesticide poisoning in the developing world–a minimum pesticides list. Lancet. 2002; 360(9340): 1163-7[PubMed]
  • 2. Karalliedde L, Senanayake N. Organophosphorus Insecticide Poisoning. Br J Anaesth. 1989; 63(6): 736-50[DOI]
  • 3. van der Hoek W, Konradsen F. Analysis of 8000 hospital admissions for acute poisoning in a rural area of Sri Lanka. Clin Toxicol (Phila). 2006; 44(3): 225-31[PubMed]
  • 4. Van der Hoek W, Konradsen F, Athukorala K, Wanigadewa T. Pesticide poisoning: a major health problem in Sri Lanka. Soc Sci Med. 1998; 46(4-5): 495-504[PubMed]
  • 5. Eddleston M, Eyer P, Worek F, Mohamed F, Senarathna L, von Meyer L, et al. Differences between organophosphorus insecticides in human self-poisoning: a prospective cohort study. Lancet. 2005; 366(9495): 1452-9[DOI][PubMed]
  • 6. Jeyaratnam J, Lun KC, Phoon WO. Survey of acute pesticide poisoning among agricultural workers in four Asian countries. Bull World Health Organ. 1987; 65(4): 521-7[PubMed]
  • 7. Calvert GM, Karnik J, Mehler L, Beckman J, Morrissey B, Sievert J, et al. Acute pesticide poisoning among agricultural workers in the United States, 1998-2005. Am J Ind Med. 2008; 51(12): 883-98[DOI][PubMed]
  • 8. Buckley NA, Karalliedde L, Dawson A, Senanayake N, Eddleston M. Where is the evidence for treatments used in pesticide poisoning? Is clinical toxicology fiddling while the developing world burns? J Toxicol Clin Toxicol. 2004; 42(1): 113-6[PubMed]
  • 9. Jeyaratnam J. Acute pesticide poisoning: a major global health problem. World Health Stat Q. 1990; 43(3): 139-44[PubMed]
  • 10. The public health im-pact of pesticides use in agriculture. 2012;
  • 11. Kesavachandran CN, Fareed M, Pathak MK, Bihari V, Mathur N, Srivastava AK. Adverse health effects of pesticides in agrarian populations of developing countries. Reviews of Environ-mental Contamination and Toxicology. 2009; : 33-52
  • 12. Abo-Amer A. Biodegradation of diazinon by Serratia marcescens DI101 and its use in bioremediation of contaminated environment. J Microbiol Biotechnol. 2011; 21(1): 71-80[PubMed]
  • 13. Arbeli Z, Fuentes CL. Accelerated biodegradation of pesticides: An overview of the phenomenon, its basis and possible solutions; and a discussion on the tropical dimension. Crop Protect. 2007; 26(12): 1733-46[DOI]
  • 14. Geetha M, Fulekar M. Bioremediation of pesticides in surface soil treatment unit using microbial consortia. African Journal of Environmental Science and Technology. 2008; 2(2): 36-45
  • 15. Caldwell SR, Newcomb JR, Schlecht KA, Raushel FM. Limits of diffusion in the hydrolysis of substrates by the phosphotriesterase from Pseudomonas diminuta. Biochemistry. 1991; 30(30): 7438-44[PubMed]
  • 16. Dumas DP, Caldwell SR, Wild JR, Raushel FM. Purification and properties of the phosphotriesterase from Pseudomonas diminuta. J Biol Chem. 1989; 264(33): 19659-65[PubMed]
  • 17. Dumas DP, Wild JR, Raushel FM. Diisoproplyfluorophosphate hydrolysis by a phosphotriesterase from Pseudomonas diminuta. Bio Technol Appl Biochem. 1989; 11: 235-43
  • 18. Siddavattam D, Khajamohiddin S, Manavathi B, Pakala SB, Merrick M. Transposon-like organization of the plasmid-borne organophosphate degradation (opd) gene cluster found in Flavobacterium sp. Appl Environ Microbiol. 2003; 69(5): 2533-9[PubMed]
  • 19. Somara S, Manavathi B, Tebbe CC, Siddavatam D. Localisation of identical organophosphorus pesticide degrading (opd) genes on genetically dissimilar indigenous plasmids of soil bacteria: PCR amplification, cloning and sequencing of opd gene from Flavobacterium balustinum. Indian J Exp Biol. 2002; 40(7): 774-9[PubMed]
  • 20. Park SC, Shimamura I, Fukunaga M, Mori KI, Nakai T. Isolation of bacteriophages specific to a fish pathogen, Pseudomonas plecoglossicida, as a candidate for disease control. Appl Environ Microbiol. 2000; 66(4): 1416-22[PubMed]
  • 21. Fulekar MH, Geetha M. Bioremediation of Chlorpyrifos by Pseudomonas aeruginosa using scale up technique. J Appl Biosci. 2008; 12: 657-60
  • 22. Cetın AK, Gur N, Fırat Z. Growth rate of Scenedesmus acutus in laboratory cultures exposed to diazinon. Afr J Biotechnol. 2011; 10(34): 6540-3
  • 23. Iyer R, Stepanov VG, Iken B. Isolation and molecular characterization of a novel <i>pseudomonas putida</i> strain capable of degrading organophosphate and aromatic compounds. Adv Biol Chem. 2013; 3(6): 564-78[DOI]
  • 24. Borji A, Naserpour Farivar G, Johari P, Naserpour Farivar T, Senemari S, Karimi G. Cleaning From the Inside: Biodegradation of Organophosphate Pesticides by Pseudomonas plecoglossicida. Biotechnol Health Sci. 2014; 1(1)[DOI]
  • 25. Mulbry WW, Karns JS. Parathion hydrolase specified by the Flavobacterium opd gene: relationship between the gene and protein. J Bacteriol. 1989; 171(12): 6740-6[PubMed]