Evaluation of MexB and MexY Genes Expression in Carbapenem-Resistant Pseudomonas aeruginosa Strains Isolated from Educational Hospitals of Qazvin and Tehran, Iran

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To Cite : Naserpour Farivar T, Peymani A, Bolori S, Rahimi H. Evaluation of MexB and MexY Genes Expression in Carbapenem-Resistant Pseudomonas aeruginosa Strains Isolated from Educational Hospitals of Qazvin and Tehran, Iran, Biotech Health Sci. 2017 ;4(2):e12922. doi: 10.5812/bhs.12922.
Abstract
1. Background
2. Methods
3. Results
4. Discussion
Acknowledgements
Footnotes
References
  • 1. Micek ST, Wunderink RG, Kollef MH, Chen C, Rello J, Chastre J, et al. An international multicenter retrospective study of Pseudomonas aeruginosa nosocomial pneumonia: impact of multidrug resistance. Crit Care. 2015; 19: 219[DOI][PubMed]
  • 2. Faraji F, Mahzounieh M, Ebrahimi A, Fallah F, Teymournejad O, Lajevardi B. Molecular detection of virulence genes in Pseudomonas aeruginosa isolated from children with Cystic Fibrosis and burn wounds in Iran. Microb Pathog. 2016; 99: 1-4[DOI][PubMed]
  • 3. Trautmann M, Lepper PM, Haller M. Ecology of Pseudomonas aeruginosa in the intensive care unit and the evolving role of water outlets as a reservoir of the organism. Am J Infect Control. 2005; 33(5 Suppl 1)-9[DOI][PubMed]
  • 4. Aloush V, Navon-Venezia S, Seigman-Igra Y, Cabili S, Carmeli Y. Multidrug-resistant Pseudomonas aeruginosa: risk factors and clinical impact. Antimicrob Agents Chemother. 2006; 50(1): 43-8[DOI][PubMed]
  • 5. Tong Y, Sun M, Wang C. Multidrug-resistant Pseudomonas aeruginosa: a case of pyelonephritis and herbal therapy. J Altern Complement Med. 2014; 20(2): 142-4[DOI][PubMed]
  • 6. Rodriguez-Martinez JM, Poirel L, Nordmann P. Molecular epidemiology and mechanisms of carbapenem resistance in Pseudomonas aeruginosa. Antimicrob Agents Chemother. 2009; 53(11): 4783-8[DOI][PubMed]
  • 7. Andrade SS, Jones RN, Gales AC, Sader HS. Increasing prevalence of antimicrobial resistance among Pseudomonas aeruginosa isolates in Latin American medical centres: 5 year report of the SENTRY Antimicrobial Surveillance Program (1997-2001). J Antimicrob Chemother. 2003; 52(1): 140-1[DOI][PubMed]
  • 8. Pao SS, Paulsen IT, Saier MJ. Major facilitator superfamily. Microbiol Mol Biol Rev. 1998; 62(1): 1-34[PubMed]
  • 9. van Veen HW, Konings WN. The ABC family of multidrug transporters in microorganisms. Biochim Biophys Acta. 1998; 1365(1-2): 31-6[PubMed]
  • 10. Nikaido H. Antibiotic resistance caused by gram-negative multidrug efflux pumps. Clin Infect Dis. 1998; 27 Suppl 1-41[PubMed]
  • 11. Saier MJ, Tam R, Reizer A, Reizer J. Two novel families of bacterial membrane proteins concerned with nodulation, cell division and transport. Mol Microbiol. 1994; 11(5): 841-7[PubMed]
  • 12. Paulsen IT, Skurray RA, Tam R, Saier MJ, Turner RJ, Weiner JH, et al. The SMR family: a novel family of multidrug efflux proteins involved with the efflux of lipophilic drugs. Mol Microbiol. 1996; 19(6): 1167-75[PubMed]
  • 13. Brown MH, Paulsen IT, Skurray RA. The multidrug efflux protein NorM is a prototype of a new family of transporters. Mol Microbiol. 1999; 31(1): 394-5[PubMed]
  • 14. Li XZ, Barre N, Poole K. Influence of the MexA-MexB-oprM multidrug efflux system on expression of the MexC-MexD-oprJ and MexE-MexF-oprN multidrug efflux systems in Pseudomonas aeruginosa. J Antimicrob Chemother. 2000; 46(6): 885-93[PubMed]
  • 15. Masuda N, Sakagawa E, Ohya S, Gotoh N, Tsujimoto H, Nishino T. Substrate specificities of MexAB-OprM, MexCD-OprJ, and MexXY-oprM efflux pumps in Pseudomonas aeruginosa. Antimicrob Agents Chemother. 2000; 44(12): 3322-7[PubMed]
  • 16. Wikler MA. Performance standards for antimicrobial susceptibility testing: Sixteenth informational supplement. 2006;
  • 17. Yoneda K, Chikumi H, Murata T, Gotoh N, Yamamoto H, Fujiwara H, et al. Measurement of Pseudomonas aeruginosa multidrug efflux pumps by quantitative real-time polymerase chain reaction. FEMS Microbiol Lett. 2005; 243(1): 125-31[DOI][PubMed]
  • 18. Shahcheraghi F, Nikbin VS, Feizabadi MM. Identification and genetic characterization of metallo-beta-lactamase-producing strains of Pseudomonas aeruginosa in Tehran, Iran. New Microbiol. 2010; 33(3): 243-8[PubMed]
  • 19. Japoni A, Alborzi A, Kalani M, Nasiri J, Hayati M, Farshad S. Susceptibility patterns and cross-resistance of antibiotics against Pseudomonas aeruginosa isolated from burn patients in the South of Iran. Burns. 2006; 32(3): 343-7[DOI][PubMed]
  • 20. Sepehriseresht S, Boroumand MA, Pourgholi L, Sotoudeh Anvari M, Habibi E, Sattarzadeh Tabrizi M. Detection of vim- and ipm-type metallo-beta-lactamases in Pseudomonas aeruginosa clinical isolates. Arch Iran Med. 2012; 15(11): 670-3[PubMed]
  • 21. Khosravi AD, Mihani F. Detection of metallo-beta-lactamase-producing Pseudomonas aeruginosa strains isolated from burn patients in Ahwaz, Iran. Diagn Microbiol Infect Dis. 2008; 60(1): 125-8[DOI][PubMed]
  • 22. Arabestani MR, Rajabpour M, Yousefi Mashouf R, Alikhani MY, Mousavi SM. Expression of efflux pump MexAB-OprM and OprD of Pseudomonas aeruginosa strains isolated from clinical samples using qRT-PCR. Arch Iran Med. 2015; 18(2): 102-8[PubMed]
  • 23. Masuda N, Sakagawa E, Ohya S, Gotoh N, Tsujimoto H, Nishino T. Contribution of the MexX-MexY-oprM efflux system to intrinsic resistance in Pseudomonas aeruginosa. Antimicrob Agents Chemother. 2000; 44(9): 2242-6[PubMed]
  • 24. Dumas JL, van Delden C, Perron K, Kohler T. Analysis of antibiotic resistance gene expression in Pseudomonas aeruginosa by quantitative real-time-PCR. FEMS Microbiol Lett. 2006; 254(2): 217-25[DOI][PubMed]
  • 25. Hocquet D, Nordmann P, El Garch F, Cabanne L, Plesiat P. Involvement of the MexXY-OprM efflux system in emergence of cefepime resistance in clinical strains of Pseudomonas aeruginosa. Antimicrob Agents Chemother. 2006; 50(4): 1347-51[DOI][PubMed]
  • 26. Terzi HA, Kulah C, Ciftci IH. The effects of active efflux pumps on antibiotic resistance in Pseudomonas aeruginosa. World J Microbiol Biotechnol. 2014; 30(10): 2681-7[DOI][PubMed]
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