Biotechnology and Health Sciences Biotechnology and Health Sciences Biotech Health Sci http://www.Biotech-health.com 2383-0271 2383-028X 10.5812/bhs en jalali 2017 5 28 gregorian 2017 5 28 S 1
en 10.5812/bhs-37475 Relationship Between Depression and Perception of Pain Severity in Patients Admitted to General Surgery Ward Relationship Between Depression and Perception of Pain Severity in Patients Admitted to General Surgery Ward research-article research-article Conclusions

Depressed or anguished patients report more pain compared to healthy ones.

Results

The highest frequency of participants had moderate depression (44.1%) while the lowest frequency belonged to healthy individuals (4.2%). The score of depression was higher in men (23.21 ± 7.56) than women (19.19 ± 6.84) as the same as the score of pain perception (8.91 ± 2.34 vs. 7.95 ± 1.87, respectively). The results indicated that there was a positive significant relationship between depression and perception of pain severity (P ≤ 0.01). This means that patients who have a history of depression feel much more pain during hospitalization and after the surgery.

Objectives

The aim of this study was to survey the relationship between depression and perception of pain severity in patients admitted to general surgery ward.

Methods

This research was a cross-sectional descriptive study. The study population included patients admitted to general surgery ward at hospitals of Ardabil city during 2010 - 2011. The study sample consisted of 168 individuals (male and female) who were selected by nonrandomized convenience sampling method. The data were collected using a questionnaire on demographic information, the Beck depression inventory (BDI), and Toren questionnaire on pain beliefs and perceptions. The SPSS software was used for statistical analysis.

Background

Depression is considered as the most common psychological problem in individuals. Patients with persistent pain usually suffer from depression, disturbance in interpersonal relations, fatigue, and reduced physical and psychological performance.

Conclusions

Depressed or anguished patients report more pain compared to healthy ones.

Results

The highest frequency of participants had moderate depression (44.1%) while the lowest frequency belonged to healthy individuals (4.2%). The score of depression was higher in men (23.21 ± 7.56) than women (19.19 ± 6.84) as the same as the score of pain perception (8.91 ± 2.34 vs. 7.95 ± 1.87, respectively). The results indicated that there was a positive significant relationship between depression and perception of pain severity (P ≤ 0.01). This means that patients who have a history of depression feel much more pain during hospitalization and after the surgery.

Objectives

The aim of this study was to survey the relationship between depression and perception of pain severity in patients admitted to general surgery ward.

Methods

This research was a cross-sectional descriptive study. The study population included patients admitted to general surgery ward at hospitals of Ardabil city during 2010 - 2011. The study sample consisted of 168 individuals (male and female) who were selected by nonrandomized convenience sampling method. The data were collected using a questionnaire on demographic information, the Beck depression inventory (BDI), and Toren questionnaire on pain beliefs and perceptions. The SPSS software was used for statistical analysis.

Background

Depression is considered as the most common psychological problem in individuals. Patients with persistent pain usually suffer from depression, disturbance in interpersonal relations, fatigue, and reduced physical and psychological performance.

Depression;Pain Perception;Surgery;General Depression;Pain Perception;Surgery;General http://www.Biotech-health.com/index.php?page=article&article_id=37475 Mehdi Samadzadeh Mehdi Samadzadeh Ardabil University of Medical Sciences, Ardabil, Iran Ardabil University of Medical Sciences, Ardabil, Iran Bita Shahbazzadegan Bita Shahbazzadegan School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran; School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran; School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran Moslem Abbasi Moslem Abbasi Department of Psychology, Faculty of Literature and Human sciences, Salman Farsi University of Kazerun, Iran Department of Psychology, Faculty of Literature and Human sciences, Salman Farsi University of Kazerun, Iran Maryam Didedar Maryam Didedar PhD in Clinical Psychology PhD in Clinical Psychology
en 10.5812/bhs-37627 Clinicopathological Findings of the Cardia and Gastroesophageal Junction in Adult Candidates for Endoscopy in Qazvin, Iran Clinicopathological Findings of the Cardia and Gastroesophageal Junction in Adult Candidates for Endoscopy in Qazvin, Iran research-article research-article Results

Helicobacter pylori were significantly related to active inflammation in the antrum and the cardia, but not in the Z-line. Another finding was that active carditis was related to active antritis and active Z-line inflammation. A further observation was the positive correlation between cardiac and Z-line metaplasia. Finally, a relationship was observed between the type of cardiac mucosa and the presence of metaplasia in the cardia and the Z-line.

Conclusions

The study revealed that inflammation in the cardia and, to a lesser extent, the Z-line is related to H. pylori and antral gastritis and that the metaplasia of these areas is related to gastroesophageal reflux disease. Lastly, both H. pylori and gastroesophageal reflux disease are important in the pathology of these areas.

Background

Chronic inflammation and intestinal metaplasia lead to gastric and esophageal adenocarcinoma. Past research has provided controversial findings about the etiology of inflammation and intestinal metaplasia in the cardia and the Z-line.

Objectives

The aim of this study was to evaluate clinicopathological findings of the cardia and gastroesophageal junction in adult candidates for endoscopy in Qazvin, Iran.

Methods

Biopsy was performed for 124 ambulatory adult patients without any evidence of cancer. Specimens were obtained from the antrum and the cardia of the stomach, the Z-line, and 2 cm above the Z-line. A comparison was made between the histological, clinical and endoscopic data obtained from the patients.

Results

Helicobacter pylori were significantly related to active inflammation in the antrum and the cardia, but not in the Z-line. Another finding was that active carditis was related to active antritis and active Z-line inflammation. A further observation was the positive correlation between cardiac and Z-line metaplasia. Finally, a relationship was observed between the type of cardiac mucosa and the presence of metaplasia in the cardia and the Z-line.

Conclusions

The study revealed that inflammation in the cardia and, to a lesser extent, the Z-line is related to H. pylori and antral gastritis and that the metaplasia of these areas is related to gastroesophageal reflux disease. Lastly, both H. pylori and gastroesophageal reflux disease are important in the pathology of these areas.

Background

Chronic inflammation and intestinal metaplasia lead to gastric and esophageal adenocarcinoma. Past research has provided controversial findings about the etiology of inflammation and intestinal metaplasia in the cardia and the Z-line.

Objectives

The aim of this study was to evaluate clinicopathological findings of the cardia and gastroesophageal junction in adult candidates for endoscopy in Qazvin, Iran.

Methods

Biopsy was performed for 124 ambulatory adult patients without any evidence of cancer. Specimens were obtained from the antrum and the cardia of the stomach, the Z-line, and 2 cm above the Z-line. A comparison was made between the histological, clinical and endoscopic data obtained from the patients.

Cardia;Z-Line;Gastroesophageal Reflux Disease;Histopathology;Helicobacter pylori Cardia;Z-Line;Gastroesophageal Reflux Disease;Histopathology;Helicobacter pylori http://www.Biotech-health.com/index.php?page=article&article_id=37627 Fatemeh Hajmanoochehri Fatemeh Hajmanoochehri Metabolic Diseases Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Metabolic Diseases Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Rasoul Samimi Rasoul Samimi Metabolic Diseases Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran; Metabolic Diseases Research Center, Booali-Sina St. Booali- Sina Hospital, Qazvin, IR Iran. Tel: +98-2833360084, +98-9121825437, Fax: +98-2833326033 Metabolic Diseases Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran; Metabolic Diseases Research Center, Booali-Sina St. Booali- Sina Hospital, Qazvin, IR Iran. Tel: +98-2833360084, +98-9121825437, Fax: +98-2833326033 Sonia Oveisi Sonia Oveisi Metabolic Diseases Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Metabolic Diseases Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Mehdi Ebtehaj Mehdi Ebtehaj Metabolic Diseases Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Metabolic Diseases Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran
en 10.5812/bhs-38592 A Cognitive Human Error Analysis with CREAM in Control Room of Petrochemical Industry A Cognitive Human Error Analysis with CREAM in Control Room of Petrochemical Industry research-article research-article Conclusions

Common Performance Conditions (CPCs), empowerment, and the time available for work were among the most important factors that reduced occupational performance. To optimize a communication system, it is necessary to arrange the priority of tasks, hold joint meetings, inform the staff about the termination of work permits, hold training sessions, and measure the pollutants.

Background

The cognitive human error analysis technique is one of the second-generation techniques used to evaluate human reliability; it has a strong, detailed theoretical background that focuses on the important cognitive features of human behavior.

Objectives

The aim of this study was to assign task and jobs crisis using analysis of cognitive human error with CREAM. Finally, based on the results, the major causes of error were detected.

Methods

This cross-sectional study was conducted on 53 people working in an olefin unit. It is one of the most important control rooms located in a special economic zone in Assaluyeh petrochemical industry. In this study, first a job analysis was conducted and the sub-tasks and conditions affecting the performance of the staff were determined. Then, the control mode coefficient and control mode type, as well as the possibility of total error were determined. Finally, the cognitive functions and type of cognitive error related to each sub-task were identified.

Results

Among the six evaluated occupational tasks, the tasks performed by board-man and site-man had the highest values of total human error in terms of transitory overall error coefficient (0.056 and 0.031, respectively). In addition, the following results were obtained on the basis of the extended CREAM: execution failure (31.72%), interpretation failure (29.20%), planning failure (14.63%), and observation failure (24.39%).

Conclusions

Common Performance Conditions (CPCs), empowerment, and the time available for work were among the most important factors that reduced occupational performance. To optimize a communication system, it is necessary to arrange the priority of tasks, hold joint meetings, inform the staff about the termination of work permits, hold training sessions, and measure the pollutants.

Background

The cognitive human error analysis technique is one of the second-generation techniques used to evaluate human reliability; it has a strong, detailed theoretical background that focuses on the important cognitive features of human behavior.

Objectives

The aim of this study was to assign task and jobs crisis using analysis of cognitive human error with CREAM. Finally, based on the results, the major causes of error were detected.

Methods

This cross-sectional study was conducted on 53 people working in an olefin unit. It is one of the most important control rooms located in a special economic zone in Assaluyeh petrochemical industry. In this study, first a job analysis was conducted and the sub-tasks and conditions affecting the performance of the staff were determined. Then, the control mode coefficient and control mode type, as well as the possibility of total error were determined. Finally, the cognitive functions and type of cognitive error related to each sub-task were identified.

Results

Among the six evaluated occupational tasks, the tasks performed by board-man and site-man had the highest values of total human error in terms of transitory overall error coefficient (0.056 and 0.031, respectively). In addition, the following results were obtained on the basis of the extended CREAM: execution failure (31.72%), interpretation failure (29.20%), planning failure (14.63%), and observation failure (24.39%).

Human Error;Control Room;Petrochemical Industry;CREAM Human Error;Control Room;Petrochemical Industry;CREAM http://www.Biotech-health.com/index.php?page=article&article_id=38592 Sana Shokria Sana Shokria Qazvin University of Medical Sciences, Qazvin, IR Iran Qazvin University of Medical Sciences, Qazvin, IR Iran Sakineh Varmazyar Sakineh Varmazyar Department of Occupational Health Engineering, Faculty of Health, Qazvin University of Medical Sciences, Qazvin, Iran; Department of Occupational Health Engineering, Faculty of Health, Qazvin University of Medical Sciences, Qazvin, Iran Department of Occupational Health Engineering, Faculty of Health, Qazvin University of Medical Sciences, Qazvin, Iran; Department of Occupational Health Engineering, Faculty of Health, Qazvin University of Medical Sciences, Qazvin, Iran Payam Heydari Payam Heydari Qazvin University of Medical Sciences, Qazvin, IR Iran Qazvin University of Medical Sciences, Qazvin, IR Iran
en 10.5812/bhs.41629 Prevalence of Anxiety and Depression in Diabetic Patients: A Comparative Study Prevalence of Anxiety and Depression in Diabetic Patients: A Comparative Study research-article research-article Conclusions

This study showed that anxiety and depression are significantly more common among diabetic patients in comparison to the control group in the city of Qazvin; therefore, it is necessary to develop primary care by a system based on the reaction, so that an effective treatment for mental health would take place and, ultimately, the impact of these interventions should be studied.

Background

Living with diabetes and managing it can have substantial emotional burden on individuals. These changes might affect individuals’ lives in terms of stress and depression. The purpose of this study was to determine the prevalence of stress and depression among diabetic women who referred to endocrine clinic of Qazvin in 2014.

Methods

For this purpose, 250 patients (125 individuals suffering from diabetes and 125 individuals as a control group) participated in this study. All individuals completed the beck depression inventory and the cattell anxiety inventory. In addition to these, demographic and clinical records were collected from their medical records and were analyzed by appropriate statistical methods.

Results

In terms of the Maximum of mild anxiety there were 52 diabetic individuals (41.6%) versus 69 individuals of the control group (55.2%); in terms of Moderate-severe anxiety there were 73 cases (58.4%) versus 56 patients (44.8%) (P value = 0.031). In studying the Maximum of mild depression, there were 43 patients (34.4%) versus 92 (73.6%); in terms of Moderate-severe depression, there were 82 patients (65.6%) versus 33 (26.4%) (P value = 0.001). On a closer examination among age, type of diabetes, duration of diabetes, and insulin injections; only the duration of having diabetes was significantly associated with depression as one of the mental health variables.

Conclusions

This study showed that anxiety and depression are significantly more common among diabetic patients in comparison to the control group in the city of Qazvin; therefore, it is necessary to develop primary care by a system based on the reaction, so that an effective treatment for mental health would take place and, ultimately, the impact of these interventions should be studied.

Background

Living with diabetes and managing it can have substantial emotional burden on individuals. These changes might affect individuals’ lives in terms of stress and depression. The purpose of this study was to determine the prevalence of stress and depression among diabetic women who referred to endocrine clinic of Qazvin in 2014.

Methods

For this purpose, 250 patients (125 individuals suffering from diabetes and 125 individuals as a control group) participated in this study. All individuals completed the beck depression inventory and the cattell anxiety inventory. In addition to these, demographic and clinical records were collected from their medical records and were analyzed by appropriate statistical methods.

Results

In terms of the Maximum of mild anxiety there were 52 diabetic individuals (41.6%) versus 69 individuals of the control group (55.2%); in terms of Moderate-severe anxiety there were 73 cases (58.4%) versus 56 patients (44.8%) (P value = 0.031). In studying the Maximum of mild depression, there were 43 patients (34.4%) versus 92 (73.6%); in terms of Moderate-severe depression, there were 82 patients (65.6%) versus 33 (26.4%) (P value = 0.001). On a closer examination among age, type of diabetes, duration of diabetes, and insulin injections; only the duration of having diabetes was significantly associated with depression as one of the mental health variables.

Mental Health;Anxiety;Depression;Diabetes Mental Health;Anxiety;Depression;Diabetes http://www.Biotech-health.com/index.php?page=article&article_id=41629 Alireza Hajseyed Javadi Alireza Hajseyed Javadi Department of Psychiatry, Qazvin University of Medical Sciences, Qazvin, IR Iran; Department of Psychiatry, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Psychiatry, Qazvin University of Medical Sciences, Qazvin, IR Iran; Department of Psychiatry, Qazvin University of Medical Sciences, Qazvin, IR Iran Amir Ziaee Amir Ziaee Department of Endocrinology, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Endocrinology, Qazvin University of Medical Sciences, Qazvin, IR Iran Zohre Yazdi Zohre Yazdi Department of Occupational Medicine, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Occupational Medicine, Qazvin University of Medical Sciences, Qazvin, IR Iran Narges Ebrahimabadi Narges Ebrahimabadi Department of Medicine, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Medicine, Qazvin University of Medical Sciences, Qazvin, IR Iran Ali Akbar Shafikhani Ali Akbar Shafikhani Department of Occupational Health, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Occupational Health, Qazvin University of Medical Sciences, Qazvin, IR Iran
en 10.5812/bhs-41877 Investigation of Effective Factors on the Quality of Life of Patients Suffering from Ulcerative Colitis in Remission Investigation of Effective Factors on the Quality of Life of Patients Suffering from Ulcerative Colitis in Remission research-article research-article Objectives

In this study, we evaluated the quality of life (QOL) of these patients in the remission period of this disease.

Methods

Health-related quality of life (HRQOL) in 96 patients suffering from UC in clinical remission phase were evaluated by the use of two questionnaires of IBDQ and SF-36 which evaluate the domains of QOL and physical-psychological health, respectively. Results were analyzed by independent sample t-test and regression analysis using of SPSS version 22.

Conclusions

In patients suffering from UC in the remission phase males had a better HRQOL compared to females. Also remission period and absence of EIM have a positive effect on the QOL of these patients.

Results

Mean total scores from SF-36 and IBDQ tests were 79.5 ± 17.7 and 48.8 ± 9.5, respectively. Based on the results of IBD-Q test, the least score was given to the systemic symptoms (11.01 ± 3.17) and the most was related to the emotional function (30.06 ± 8.03). In the domain of physical-psychological health, physical health (15.06 ± 25.7) compared to the psychological health (33.5 ± 7.8) had a higher score. In addition, the mean total score from the IBD-Q test (P = 0.017), intestinal symptoms (P = 0.015) and emotional function (P = 0.007) were statistically more significant in males compared to the females.

Background

Ulcerative colitis (UC) is a chronic inflammatory disease with a variable and unpredictable course. This disease has noticeable consequences and by causing limitations in the life style of patients affects their quality of life.

Objectives

In this study, we evaluated the quality of life (QOL) of these patients in the remission period of this disease.

Methods

Health-related quality of life (HRQOL) in 96 patients suffering from UC in clinical remission phase were evaluated by the use of two questionnaires of IBDQ and SF-36 which evaluate the domains of QOL and physical-psychological health, respectively. Results were analyzed by independent sample t-test and regression analysis using of SPSS version 22.

Conclusions

In patients suffering from UC in the remission phase males had a better HRQOL compared to females. Also remission period and absence of EIM have a positive effect on the QOL of these patients.

Results

Mean total scores from SF-36 and IBDQ tests were 79.5 ± 17.7 and 48.8 ± 9.5, respectively. Based on the results of IBD-Q test, the least score was given to the systemic symptoms (11.01 ± 3.17) and the most was related to the emotional function (30.06 ± 8.03). In the domain of physical-psychological health, physical health (15.06 ± 25.7) compared to the psychological health (33.5 ± 7.8) had a higher score. In addition, the mean total score from the IBD-Q test (P = 0.017), intestinal symptoms (P = 0.015) and emotional function (P = 0.007) were statistically more significant in males compared to the females.

Background

Ulcerative colitis (UC) is a chronic inflammatory disease with a variable and unpredictable course. This disease has noticeable consequences and by causing limitations in the life style of patients affects their quality of life.

Ulcerative Colitis;Health Related Quality of Life;IBDQ;SF-36 Ulcerative Colitis;Health Related Quality of Life;IBDQ;SF-36 http://www.Biotech-health.com/index.php?page=article&article_id=41877 Ali Akbar Hajaghamohammadi Ali Akbar Hajaghamohammadi Department of Internal Medicine,Velayat Clinical Research Development Unit,Velayat Hospital, Qazvin University of Medical Sciences, Qazvin, Iran Department of Internal Medicine,Velayat Clinical Research Development Unit,Velayat Hospital, Qazvin University of Medical Sciences, Qazvin, Iran Bahareh Keshavarzi Bahareh Keshavarzi Velayat Clinical Research Development Unit, Velayat Hospital, Qazvin University of Medical Sciences, Qazvin, Iran Velayat Clinical Research Development Unit, Velayat Hospital, Qazvin University of Medical Sciences, Qazvin, Iran Ali Zargar Ali Zargar Department of Internal Medicine,Velayat Clinical Research Development Unit,Velayat Hospital, Qazvin University of Medical Sciences, Qazvin, Iran; Department of Internal Medicine,Velayat Clinical Research Development Unit,Velayat Hospital, Qazvin University of Medical Sciences, Qazvin, Iran. Tel: +98-2833760620 Department of Internal Medicine,Velayat Clinical Research Development Unit,Velayat Hospital, Qazvin University of Medical Sciences, Qazvin, Iran; Department of Internal Medicine,Velayat Clinical Research Development Unit,Velayat Hospital, Qazvin University of Medical Sciences, Qazvin, Iran. Tel: +98-2833760620
en 10.5812/bhs-43712 Simultaneous Detection of Pathogenic and Saprophyte Leptospira in Human Plasma by Multiplex Taqman Real Time PCR Simultaneous Detection of Pathogenic and Saprophyte Leptospira in Human Plasma by Multiplex Taqman Real Time PCR research-article research-article Background

Leptospirosis is an infectious disease caused by pathogenic and saprophytic Leptospira species. The clinical and laboratory diagnosis of this infection is complicated. However, timely diagnosis of leptospirosis is essential for treatment of this disease. Conventional laboratory methods are incapable in the early diagnosis of it. Molecular tests such as real time PCR are very efficient when diagnosing it.

Results

Of the 250 samples, 93 (37.2%) were positive for pathogenic and 15 (6%) for non-pathogenic cases. In two samples, pathogenic and non-pathogenic DNA strains were simultaneously positive.

Conclusions

Based on our finds, the real time PCR is a suitable test for the diagnosis of leptospirosis and differentiation between pathogen and saprophyte Leptospira simultaneously.

Methods

250 human plasma samples were obtained from suspected patients. Two pair specific primers and the corresponding probe for detecting pathogenic and saprophytic Leptospira were designed and established in a single tube. The developed tests were run on all DNA extracted from the samples.

Objectives

In this study, we designed and developed a multiplex Taqman real time PCR to simultaneously detect saprophyte and pathologic Leptospira in clinical samples.

Background

Leptospirosis is an infectious disease caused by pathogenic and saprophytic Leptospira species. The clinical and laboratory diagnosis of this infection is complicated. However, timely diagnosis of leptospirosis is essential for treatment of this disease. Conventional laboratory methods are incapable in the early diagnosis of it. Molecular tests such as real time PCR are very efficient when diagnosing it.

Results

Of the 250 samples, 93 (37.2%) were positive for pathogenic and 15 (6%) for non-pathogenic cases. In two samples, pathogenic and non-pathogenic DNA strains were simultaneously positive.

Conclusions

Based on our finds, the real time PCR is a suitable test for the diagnosis of leptospirosis and differentiation between pathogen and saprophyte Leptospira simultaneously.

Methods

250 human plasma samples were obtained from suspected patients. Two pair specific primers and the corresponding probe for detecting pathogenic and saprophytic Leptospira were designed and established in a single tube. The developed tests were run on all DNA extracted from the samples.

Objectives

In this study, we designed and developed a multiplex Taqman real time PCR to simultaneously detect saprophyte and pathologic Leptospira in clinical samples.

Leptospira;Real Time PCR;Saprophyte;Leptospirosis Leptospira;Real Time PCR;Saprophyte;Leptospirosis http://www.Biotech-health.com/index.php?page=article&article_id=43712 Safar Ali Alizadeh Safar Ali Alizadeh Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Amir Javadi Amir Javadi School of Allied Sciences, Tehran University of Medical Sciences, Tehran, IR Iran School of Allied Sciences, Tehran University of Medical Sciences, Tehran, IR Iran Sajjad Alizadeh Sajjad Alizadeh Tehran University of Medical Sciences, Tehran, IR Iran Tehran University of Medical Sciences, Tehran, IR Iran Reza Najafipour Reza Najafipour Department of Biochemistry, Genetics Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran; Department of Biochemistry, Genetics Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel/Fax: +98-2833324971 Department of Biochemistry, Genetics Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran; Department of Biochemistry, Genetics Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel/Fax: +98-2833324971 Taghi Naserpour Farivar Taghi Naserpour Farivar Department of Microbiology, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran; Department of Microbiology, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel/Fax: +98-2833324971 Department of Microbiology, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran; Department of Microbiology, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel/Fax: +98-2833324971
en 10.5812/bhs.45667 Capsid Modified Bluetongue Virus 16 (BTV16) as a Virulytic Oncotherapy Agent Capsid Modified Bluetongue Virus 16 (BTV16) as a Virulytic Oncotherapy Agent research-article research-article Conclusions

Modified VP2 BTV16 may be used as a potential virulytic oncotherapy agent in AGS cells.

Objective

Using potential viruses to destroy cancer cells has a long history, but recent advances in molecular biology raised hopes for successful use of these viruses again.

Methods

Octreotate sequence was inserted into the neutralization region (R1& R2) in vp2 protein of capsid segment in 10 segmented genome of BTV in 304 - 368 position. T7 BTV RNA transcripts were extracted. Cancerous cultured cells were transfected with wild and modified BTV to recover BTV with cDNA-derived genome segments.

Results

The results of all the performed experiments revealed that treatment of AGS cell lines with VP2 modified BTV16, which targeted cell surface of cancerous cells, significantly increased apoptosis in cancer infected cells.

Conclusions

Modified VP2 BTV16 may be used as a potential virulytic oncotherapy agent in AGS cells.

Objective

Using potential viruses to destroy cancer cells has a long history, but recent advances in molecular biology raised hopes for successful use of these viruses again.

Methods

Octreotate sequence was inserted into the neutralization region (R1& R2) in vp2 protein of capsid segment in 10 segmented genome of BTV in 304 - 368 position. T7 BTV RNA transcripts were extracted. Cancerous cultured cells were transfected with wild and modified BTV to recover BTV with cDNA-derived genome segments.

Results

The results of all the performed experiments revealed that treatment of AGS cell lines with VP2 modified BTV16, which targeted cell surface of cancerous cells, significantly increased apoptosis in cancer infected cells.

Bluetongue Virus;Oncotherapy;Virulytic;AGS Bluetongue Virus;Oncotherapy;Virulytic;AGS http://www.Biotech-health.com/index.php?page=article&article_id=45667 Taghi Naserpour Farivar Taghi Naserpour Farivar Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran; Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel: +98-283324971 Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran; Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel: +98-283324971 Reza Najafipour Reza Najafipour Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Safar Ali Alizadeh Safar Ali Alizadeh Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Seyyed Mahmoud Azimi Seyyed Mahmoud Azimi Razi Vaccine and Serum Research Institute, Karaj, IR Iran Razi Vaccine and Serum Research Institute, Karaj, IR Iran Pouran Johari Pouran Johari Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran
en 10.5812/bhs-39182 Iranian Traditional Medicine as a Science Not Folkloric Medicine Iranian Traditional Medicine as a Science Not Folkloric Medicine letter letter http://www.Biotech-health.com/index.php?page=article&article_id=39182 Marzieh Beygom Siahpoosh Marzieh Beygom Siahpoosh Council of Iranian Traditional Medicine, Qazvin University of Medical Sciences, Qazvin, IR Iran; Council of Iranian Traditional Medicine, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel: +98-2833332935, Fax: +98-2833356696 Council of Iranian Traditional Medicine, Qazvin University of Medical Sciences, Qazvin, IR Iran; Council of Iranian Traditional Medicine, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel: +98-2833332935, Fax: +98-2833356696