Biotechnology and Health Sciences Biotechnology and Health Sciences Biotech Health Sci http://www.Biotech-health.com 2383-0271 2383-028X 10.5812/bhs en jalali 2017 5 28 gregorian 2017 5 28 2 4
en 10.17795/bhs-32635 Inhibitory Activity of Artemisia spicigera Essential Oil Against Fungal Species Isolated From Minced Meat Inhibitory Activity of <italic>Artemisia</italic> <italic>spicigera </italic> Essential Oil Against Fungal Species Isolated From Minced Meat research-article research-article Conclusions

The results of the present study show a favorable inhibitory effect of Artemisias spicigera essential oil on fungal growth, especially Aspergillus species. According to the results, antifungal components of Artemisias spicigera in different forms are used to prevent fungal pollution.

Materials and Methods

Two types of media dichloran 18% glycerol (DG18) agar and dichloran rosebengal chloramphenicol (DRBC) agar were selected for the mycological analysis of the minced meat samples. To evaluate the antifungal activity of essential oils, the microdilution broth method based on the CLSI (M27A) guideline was used.

Results

Artemisias spicigera essential oil has an inhibitory effect on the growth of fungi found in samples of minced meat. Aspergillus, Penicillium and Cladosporium were the most common genera on both medium types. Average Minimum Inhibitory Concentration 50 = 1.88 µL/mL and MIC90 = 2 µL/mL were reported. The genus of Mucor with MIC = 1.0 µL/mL was the most sensitive and Aspergilus versicolor was the most resistant species to the essential oil with MIC = 4 µL/mL.

Background

Meat is an important source of several nutrients. The capability top of fresh meat to rot, causing the group of studies food science, biological and chemical stability meat consideration.

Objectives

This study was conducted to examine the inhibitory effect of Artemisias spicigera essential oil against fungal species isolated from minced meat.

Conclusions

The results of the present study show a favorable inhibitory effect of Artemisias spicigera essential oil on fungal growth, especially Aspergillus species. According to the results, antifungal components of Artemisias spicigera in different forms are used to prevent fungal pollution.

Materials and Methods

Two types of media dichloran 18% glycerol (DG18) agar and dichloran rosebengal chloramphenicol (DRBC) agar were selected for the mycological analysis of the minced meat samples. To evaluate the antifungal activity of essential oils, the microdilution broth method based on the CLSI (M27A) guideline was used.

Results

Artemisias spicigera essential oil has an inhibitory effect on the growth of fungi found in samples of minced meat. Aspergillus, Penicillium and Cladosporium were the most common genera on both medium types. Average Minimum Inhibitory Concentration 50 = 1.88 µL/mL and MIC90 = 2 µL/mL were reported. The genus of Mucor with MIC = 1.0 µL/mL was the most sensitive and Aspergilus versicolor was the most resistant species to the essential oil with MIC = 4 µL/mL.

Background

Meat is an important source of several nutrients. The capability top of fresh meat to rot, causing the group of studies food science, biological and chemical stability meat consideration.

Objectives

This study was conducted to examine the inhibitory effect of Artemisias spicigera essential oil against fungal species isolated from minced meat.

Antifungal Agent;Food Contamination;Meat;Artemisia Antifungal Agent;Food Contamination;Meat;Artemisia http://www.Biotech-health.com/index.php?page=article&article_id=32635 Peyman Ghajarbeygi Peyman Ghajarbeygi Department of Public Health, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Public Health, Qazvin University of Medical Sciences, Qazvin, IR Iran Nargess Saki Nargess Saki Department of Public Health, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Public Health, Qazvin University of Medical Sciences, Qazvin, IR Iran Farzad Katiraee Farzad Katiraee Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, IR Iran Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, IR Iran Razzagh Mahmoudi Razzagh Mahmoudi Department of Public Health, Qazvin University of Medical Sciences, Qazvin, IR Iran; Department of Public Health, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel: +98-9127868571 Department of Public Health, Qazvin University of Medical Sciences, Qazvin, IR Iran; Department of Public Health, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel: +98-9127868571
en 10.17795/bhs-31211 Screen Time and Physical Activity in Overweight and Obese Students Screen Time and Physical Activity in Overweight and Obese Students research-article research-article Conclusions

Our research determined an association between screen time, physical activity and RBMI. Screen time was a prominent predictor of RBMI in children. It is important for health workers to decrease screen time of children to prevent the prevalence of being overweight and obesity.

Patients and Methods

In this cross sectional descriptive study we randomly selected 302 students, from all districts of Qazvin, who’s relative body mass index (RBMIs) were above the 85th percentile. Their screen time and physical activity prevalence were assessed with two separate techniques (self-report and parent-report). Pearson correlation test and regression analysis were done to examine the association between RBMI, screen time and physical activity.

Results

Mean screen time in boys was more than girls, in both overweight screen time- self report technique (ST-SRT: 1.93 ± 0.24 vs. 1.26 ± 0.44, ST-PRT: 3.4 ± 0.22 vs. 2.1 ± 0.15) and obese subjects (ST-SRT: 1.88 ± 0.31 vs. 1.37 ± 0.49, ST-PRT: 3.2 ± 0.32 vs. 2.3 ± 0.34) yet overweight subjects had less total screen time than obese individuals (P < 0.05). Pearson correlations with one-tailed test indicated that screen time had a significant association with RBMI. In addition, there was a significant association between the two techniques of screen time and physical activity measurements. The model of regression for screen time and RBMI was significant (F = 45, P = 0.000, R2 = 0.42) and screen time explained about 16% of variance in RBMI (B = 0.021, SEB = 0.004, β = 0.325).

Background

One of the most important threats for children’s health status is being overweight and obesity, and related causes such as screen time prevalence. Prevalence of being overweight and obesity in children is associated with health risk consequences in adulthood.

Objectives

The purpose of this study was to assess the prevalence of screen time and physical activity in overweight and obese students.

Conclusions

Our research determined an association between screen time, physical activity and RBMI. Screen time was a prominent predictor of RBMI in children. It is important for health workers to decrease screen time of children to prevent the prevalence of being overweight and obesity.

Patients and Methods

In this cross sectional descriptive study we randomly selected 302 students, from all districts of Qazvin, who’s relative body mass index (RBMIs) were above the 85th percentile. Their screen time and physical activity prevalence were assessed with two separate techniques (self-report and parent-report). Pearson correlation test and regression analysis were done to examine the association between RBMI, screen time and physical activity.

Results

Mean screen time in boys was more than girls, in both overweight screen time- self report technique (ST-SRT: 1.93 ± 0.24 vs. 1.26 ± 0.44, ST-PRT: 3.4 ± 0.22 vs. 2.1 ± 0.15) and obese subjects (ST-SRT: 1.88 ± 0.31 vs. 1.37 ± 0.49, ST-PRT: 3.2 ± 0.32 vs. 2.3 ± 0.34) yet overweight subjects had less total screen time than obese individuals (P < 0.05). Pearson correlations with one-tailed test indicated that screen time had a significant association with RBMI. In addition, there was a significant association between the two techniques of screen time and physical activity measurements. The model of regression for screen time and RBMI was significant (F = 45, P = 0.000, R2 = 0.42) and screen time explained about 16% of variance in RBMI (B = 0.021, SEB = 0.004, β = 0.325).

Background

One of the most important threats for children’s health status is being overweight and obesity, and related causes such as screen time prevalence. Prevalence of being overweight and obesity in children is associated with health risk consequences in adulthood.

Objectives

The purpose of this study was to assess the prevalence of screen time and physical activity in overweight and obese students.

Physical Activity;Sedentary Behavior;Overweight;Obesity Physical Activity;Sedentary Behavior;Overweight;Obesity http://www.Biotech-health.com/index.php?page=article&article_id=31211 Kazem Hosseinzadeh Kazem Hosseinzadeh Nursing Department, Faculty of Nursing and Midwifery, Qazvin University of Medical Sciences, Qazvin, IR Iran; Nursing Department, Faculty of Nursing and Midwifery, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel: +98-9112325786, Fax: +98-2833237868 Nursing Department, Faculty of Nursing and Midwifery, Qazvin University of Medical Sciences, Qazvin, IR Iran; Nursing Department, Faculty of Nursing and Midwifery, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel: +98-9112325786, Fax: +98-2833237868 Mostafa Shokati Ahmadabad Mostafa Shokati Ahmadabad Nursing Department, Faculty of Nursing and Midwifery, Qazvin University of Medical Sciences, Qazvin, IR Iran Nursing Department, Faculty of Nursing and Midwifery, Qazvin University of Medical Sciences, Qazvin, IR Iran
en 10.17795/bhs-32605 Artemisia spicigera Essential Oil: Assessment of Phytochemical and Antioxidant Properties <italic>Artemisia spicigera</italic> Essential Oil: Assessment of Phytochemical and Antioxidant Properties research-article research-article Conclusions

Results have shown that A. spicigera EO before and after flowering has antioxidant properties and therefore can be used in combination with other preservatives to protect food materials against a variety of oxidative systems.

Background

Essential oils (EO), also called volatile odoriferous oil, are aromatic oily liquids extracted from different parts of plants. In general, the constituents in EOs are terpenes, aromatic compounds (aldehyde, alcohol, phenol, methoxy derivatives, and so on), and terpenoids (isoprenoids). Essential Oils have been known to possess antioxidant and antimicrobial activities, thereby serving as natural additives in foods and food products.

Results

Analysis of A. spicigera EO by gas chromatogram-mass spectrometry showed that spachulenol 1 H cycloprop (18.39%) and bicyclo hexan-3-en, 4-met (26.16%), were the prominent EOs of Artemisia before and after the flowering stage; the total phenolic EO before and after the flowering stage was 23.61 ± 1.08 µg/mL and 17.71 ± 0.9 µg/mL, respectively. Also level of flavonoid content before and after the flowering stage was 37.27 ± 1.70 µg/mL and 29.04 ± 1.30 µg/mL, respectively. This EO was able to reduce the stable free radical 2, 2-diphenol,1-picryl hydrazyl (DPPH) with an IC50 of 86.14 ± 2.23 and 96.18 ± 2.61 µg/mL, before and after flowering, respectively. Yield of EO before and after flowering was 0.5% and 0.6%, respectively.

Materials and Methods

A. spicigera EO was analyzed by gas chromatogram/mass spectrometry (GC-MS). The antioxidant activity and total phenolic content before and after flowering were evaluated by the Folin Ciocalteu method. Also, the yields of essential oil as a percentage based on the level of dry plant and the volume of extracted oil was determined.

Objectives

The aim of this study was to assess the quantity and quality of compounds, with active chemical and antioxidant properties, of Artemisia spicigera essential oil (EO) due to the effect of geographic location and season of harvest on the phenolic compounds of the plant. The plant was collected from east Azarbayjan province, Iran (both before and after the flowering stage).

Conclusions

Results have shown that A. spicigera EO before and after flowering has antioxidant properties and therefore can be used in combination with other preservatives to protect food materials against a variety of oxidative systems.

Background

Essential oils (EO), also called volatile odoriferous oil, are aromatic oily liquids extracted from different parts of plants. In general, the constituents in EOs are terpenes, aromatic compounds (aldehyde, alcohol, phenol, methoxy derivatives, and so on), and terpenoids (isoprenoids). Essential Oils have been known to possess antioxidant and antimicrobial activities, thereby serving as natural additives in foods and food products.

Results

Analysis of A. spicigera EO by gas chromatogram-mass spectrometry showed that spachulenol 1 H cycloprop (18.39%) and bicyclo hexan-3-en, 4-met (26.16%), were the prominent EOs of Artemisia before and after the flowering stage; the total phenolic EO before and after the flowering stage was 23.61 ± 1.08 µg/mL and 17.71 ± 0.9 µg/mL, respectively. Also level of flavonoid content before and after the flowering stage was 37.27 ± 1.70 µg/mL and 29.04 ± 1.30 µg/mL, respectively. This EO was able to reduce the stable free radical 2, 2-diphenol,1-picryl hydrazyl (DPPH) with an IC50 of 86.14 ± 2.23 and 96.18 ± 2.61 µg/mL, before and after flowering, respectively. Yield of EO before and after flowering was 0.5% and 0.6%, respectively.

Materials and Methods

A. spicigera EO was analyzed by gas chromatogram/mass spectrometry (GC-MS). The antioxidant activity and total phenolic content before and after flowering were evaluated by the Folin Ciocalteu method. Also, the yields of essential oil as a percentage based on the level of dry plant and the volume of extracted oil was determined.

Objectives

The aim of this study was to assess the quantity and quality of compounds, with active chemical and antioxidant properties, of Artemisia spicigera essential oil (EO) due to the effect of geographic location and season of harvest on the phenolic compounds of the plant. The plant was collected from east Azarbayjan province, Iran (both before and after the flowering stage).

Essential Oils;Antioxidants;Gas Chromatography;Artemisia spicigera Essential Oils;Antioxidants;Gas Chromatography;Artemisia spicigera http://www.Biotech-health.com/index.php?page=article&article_id=32605 Peyman Ghajarbeygi Peyman Ghajarbeygi Department of Public Health, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Public Health, Qazvin University of Medical Sciences, Qazvin, IR Iran Azar Mohammadi Azar Mohammadi Department of Public Health, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Public Health, Qazvin University of Medical Sciences, Qazvin, IR Iran Razzagh Mahmoudi Razzagh Mahmoudi Department of Public Health, Qazvin University of Medical Sciences, Qazvin, IR Iran; Department of Public Health, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel: +98-9127868571 Department of Public Health, Qazvin University of Medical Sciences, Qazvin, IR Iran; Department of Public Health, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel: +98-9127868571 Morteza Kosari-Nasab Morteza Kosari-Nasab Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, IR Iran Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, IR Iran
en 10.17795/bhs-31666 Normal Saline and Dextrose-Saline Infusion Comparison in the Duration of Active Phase in Nulliparous Women Normal Saline and Dextrose-Saline Infusion Comparison in the Duration of Active Phase in Nulliparous Women research-article research-article Conclusions

The administration of dextrose saline was associated with a shortened active phase and second stage of labor in vaginally delivered nulliparous women.

Results

Of 174 objects enrolled, 150 women delivered vaginally and completed the study. There was a significant difference in the duration of active phase between the groups (NS: 270.20 ± 13.37 minutes; D5NS: 206.67 ± 11.72 minutes) (P < 0.001) and second stage of labor (NS: 45.20 ± 1.65 minutes; D5NS: 37.27 ± 1.73 minutes) (P < 0.001). No significant difference was observed in the need to oxytocin as well as in Apgar score between the groups.

Background

During labor, it is not unusual for women to have little or no nutrient intake, in spite of the fact that the demand for energy increases as a result of skeletal and smooth muscle contractions.

Objectives

The objective of the study was to compare the effects of intravenous normal saline with dextrose-saline on the duration of active phase in nulliparous women.

Patients and Methods

In this randomized clinical trial, 174 nulliparous women with gestational age of ≥ 36 weeks in the active phase of spontaneous labor were divided into two groups, receiving either normal saline (NS) or dextrose 5% in normal saline (D5NS) with a rate of 120 mL/hour. The primary outcome was the duration of active phase of labor in vaginally-delivered subjects. The secondary outcomes were the duration of second stage of labor, need to oxytocin, and neonate Apgar score. Data were analyzed with t-test and chi-squared test.

Conclusions

The administration of dextrose saline was associated with a shortened active phase and second stage of labor in vaginally delivered nulliparous women.

Results

Of 174 objects enrolled, 150 women delivered vaginally and completed the study. There was a significant difference in the duration of active phase between the groups (NS: 270.20 ± 13.37 minutes; D5NS: 206.67 ± 11.72 minutes) (P < 0.001) and second stage of labor (NS: 45.20 ± 1.65 minutes; D5NS: 37.27 ± 1.73 minutes) (P < 0.001). No significant difference was observed in the need to oxytocin as well as in Apgar score between the groups.

Background

During labor, it is not unusual for women to have little or no nutrient intake, in spite of the fact that the demand for energy increases as a result of skeletal and smooth muscle contractions.

Objectives

The objective of the study was to compare the effects of intravenous normal saline with dextrose-saline on the duration of active phase in nulliparous women.

Patients and Methods

In this randomized clinical trial, 174 nulliparous women with gestational age of ≥ 36 weeks in the active phase of spontaneous labor were divided into two groups, receiving either normal saline (NS) or dextrose 5% in normal saline (D5NS) with a rate of 120 mL/hour. The primary outcome was the duration of active phase of labor in vaginally-delivered subjects. The secondary outcomes were the duration of second stage of labor, need to oxytocin, and neonate Apgar score. Data were analyzed with t-test and chi-squared test.

Dextrose;Hydration;Labor;Nulliparity Dextrose;Hydration;Labor;Nulliparity http://www.Biotech-health.com/index.php?page=article&article_id=31666 Farideh Movahed Farideh Movahed Department of Obstetrics and Gynecology, Faculty of Medicine, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Obstetrics and Gynecology, Faculty of Medicine, Qazvin University of Medical Sciences, Qazvin, IR Iran Hamideh Pakniat Hamideh Pakniat Department of Obstetrics and Gynecology, Faculty of Medicine, Qazvin University of Medical Sciences, Qazvin, IR Iran; Department of Obstetrics and Gynecology, Faculty of Medicine, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel: +98-9121822448, Fax: +98-2833242661 Department of Obstetrics and Gynecology, Faculty of Medicine, Qazvin University of Medical Sciences, Qazvin, IR Iran; Department of Obstetrics and Gynecology, Faculty of Medicine, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel: +98-9121822448, Fax: +98-2833242661 Mina Ataee Mina Ataee Department of Obstetrics and Gynecology, Faculty of Medicine, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Obstetrics and Gynecology, Faculty of Medicine, Qazvin University of Medical Sciences, Qazvin, IR Iran Ameneh Barikani Ameneh Barikani Children Growth Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Children Growth Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Laleh Jamsi Laleh Jamsi Department of Obstetrics and Gynecology, Faculty of Medicine, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Obstetrics and Gynecology, Faculty of Medicine, Qazvin University of Medical Sciences, Qazvin, IR Iran
en 10.17795/bhs-33196 Hospital Managers’ Perception of Recent Health Care Reform in Teaching Hospitals of Qazvin, Iran Hospital Managers’ Perception of Recent Health Care Reform in Teaching Hospitals of Qazvin, Iran research-article research-article Conclusions

The findings showed that based on the managers’ point of view, the reform plan was able to achieve its primary goals; however, it could not meet their exceptions in improving the society health status. Therefore, it is necessary to design some interventions for changing this perception.

Results

A total of 43.2% of managers believed that this reform was a good restrictor for malpractices in healthcare and 31.8% believed that it will not be so useful to improve the society health status. The average score of resource preparation, insurance companies coordination, changing the routine workflows, and finally achieving the goals, had a meaningful difference (P ˂ 0.05) and the average score of these fields were upper than average.

Patients and Methods

This cross-sectional study was conducted in January 2015. The statistical population consisted of 50 executive managers of Qazvin teaching hospitals. The data gathering instrument was a research-made questionnaire with approved reliability and validity (α = 0.84). Data analyse was performed in SPSS version 20 using descriptive and analytic statistics (analysis of variance (ANOVA), Pearson correlation test and one sample t-test).

Background

The main purpose of any government from a healthcare reform is to improve the service quality and raised public satisfaction.

Objectives

As the important role of managerial human resources in any organizational changes, this paper tried to examine the point of view of this group about the recent reform in governmental hospitals of Qazvin.

Conclusions

The findings showed that based on the managers’ point of view, the reform plan was able to achieve its primary goals; however, it could not meet their exceptions in improving the society health status. Therefore, it is necessary to design some interventions for changing this perception.

Results

A total of 43.2% of managers believed that this reform was a good restrictor for malpractices in healthcare and 31.8% believed that it will not be so useful to improve the society health status. The average score of resource preparation, insurance companies coordination, changing the routine workflows, and finally achieving the goals, had a meaningful difference (P ˂ 0.05) and the average score of these fields were upper than average.

Patients and Methods

This cross-sectional study was conducted in January 2015. The statistical population consisted of 50 executive managers of Qazvin teaching hospitals. The data gathering instrument was a research-made questionnaire with approved reliability and validity (α = 0.84). Data analyse was performed in SPSS version 20 using descriptive and analytic statistics (analysis of variance (ANOVA), Pearson correlation test and one sample t-test).

Background

The main purpose of any government from a healthcare reform is to improve the service quality and raised public satisfaction.

Objectives

As the important role of managerial human resources in any organizational changes, this paper tried to examine the point of view of this group about the recent reform in governmental hospitals of Qazvin.

Healthcare Reform;Managers;Teaching Hospitals Healthcare Reform;Managers;Teaching Hospitals http://www.Biotech-health.com/index.php?page=article&article_id=33196 Mohammad Zakaria Kiaei Mohammad Zakaria Kiaei Department of Health Service Management, School of Public Health, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Health Service Management, School of Public Health, Qazvin University of Medical Sciences, Qazvin, IR Iran Reza Moradi Reza Moradi Department of Health Services Management, Isfahan University of Medical Sciences, Isfahan, IR Iran Department of Health Services Management, Isfahan University of Medical Sciences, Isfahan, IR Iran Edris Hasanpoor Edris Hasanpoor Iranian Center of Excellence in Health Management, Tabriz University of Medical Sciences, Tabriz, IR Iran Iranian Center of Excellence in Health Management, Tabriz University of Medical Sciences, Tabriz, IR Iran Mahan Mohammadi Mahan Mohammadi Department of Health Services Management, Isfahan University of Medical Sciences, Isfahan, IR Iran Department of Health Services Management, Isfahan University of Medical Sciences, Isfahan, IR Iran Anis Taheri Anis Taheri Department of Health Service Management, School of Public Health, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Health Service Management, School of Public Health, Qazvin University of Medical Sciences, Qazvin, IR Iran Mahdieh Sadat Ahmadzadeh Mahdieh Sadat Ahmadzadeh Health Human Resource Research Center, School of Management and Information Sciences, Shiraz University of Medical Sciences, Shiraz, IR Iran; Health Human Resource Research Center, School of Management and Information Sciences, Shiraz University of Medical Sciences, Shiraz, IR Iran. Tel: +98-9337822884, Fax: +98-7132340775 Health Human Resource Research Center, School of Management and Information Sciences, Shiraz University of Medical Sciences, Shiraz, IR Iran; Health Human Resource Research Center, School of Management and Information Sciences, Shiraz University of Medical Sciences, Shiraz, IR Iran. Tel: +98-9337822884, Fax: +98-7132340775
en 10.17795/bhs-33684 The Role of Amnion Membrane-Derived Mesenchymal Stem Cells on Differentiation and Expansion of Natural Killer Cell Progenitors Originated From Umbilical Cord Blood Mononuclear Cells The Role of Amnion Membrane-Derived Mesenchymal Stem Cells on Differentiation and Expansion of Natural Killer Cell Progenitors Originated From Umbilical Cord Blood Mononuclear Cells research-article research-article Conclusions

Our results suggested that CB-NK cells progenitors could proliferate and differentiate on feeder layer of amnion membrane MSCs in combination with specific cytokines to produce NK cells for immunotherapy.

Results

Combination of cytokines and MSC layer yielded significant fold expansion in comparison with cytokines without feeder conditions (day 7: 5.2 ± 1.12 and 2 ± 0.78, respectively, P < 0.05). CD3-/CD56+ cells percentage increased during the culture period in MSCs/with cytokine and cytokine/without feeder, respectively (day 0: 4.4 ± 0.42% and day 7: 22.9 ± 3.6% and 13.9 ± 1.92 % for MSC/with cytokine and cytokine without feeder, respectively).

Objectives

The aim of this study was to make an optimum in vitro condition to proliferate and differentiate cord blood (CB)-NK cell progenitors to mature NK cells, which can be used for cell therapy.

Materials and Methods

In our study, CB-Mononuclear Cells’ (MNCs) CD3+ lymphocytes were positive depleted using immunomagnetic microbeads. This CD3-depleted (CD3-dep) CB - MNCs compartment was used for in vitro expansion with or without a layer of amnion membrane mesenchymal stem cells (MSCs) in combination with cytokines that are essential for NK cells expansion (IL-2, IL-3, IL-15, and FLT3 ligand). The expansion period lasted for one week. On day seven, immunophenotype and fold expansion of differentiated cells were measured.

Background

Natural killer (NK) cells are members of the innate immune system. Their unique properties, including recognition of viral infected and tumor cells without major histocompatibility complex (MHC) restriction or prior sensitization, make them a suitable choice for immunotherapy. Low numbers of NK cells in circulating blood is the most important obstacle for this goal.

Conclusions

Our results suggested that CB-NK cells progenitors could proliferate and differentiate on feeder layer of amnion membrane MSCs in combination with specific cytokines to produce NK cells for immunotherapy.

Results

Combination of cytokines and MSC layer yielded significant fold expansion in comparison with cytokines without feeder conditions (day 7: 5.2 ± 1.12 and 2 ± 0.78, respectively, P < 0.05). CD3-/CD56+ cells percentage increased during the culture period in MSCs/with cytokine and cytokine/without feeder, respectively (day 0: 4.4 ± 0.42% and day 7: 22.9 ± 3.6% and 13.9 ± 1.92 % for MSC/with cytokine and cytokine without feeder, respectively).

Objectives

The aim of this study was to make an optimum in vitro condition to proliferate and differentiate cord blood (CB)-NK cell progenitors to mature NK cells, which can be used for cell therapy.

Materials and Methods

In our study, CB-Mononuclear Cells’ (MNCs) CD3+ lymphocytes were positive depleted using immunomagnetic microbeads. This CD3-depleted (CD3-dep) CB - MNCs compartment was used for in vitro expansion with or without a layer of amnion membrane mesenchymal stem cells (MSCs) in combination with cytokines that are essential for NK cells expansion (IL-2, IL-3, IL-15, and FLT3 ligand). The expansion period lasted for one week. On day seven, immunophenotype and fold expansion of differentiated cells were measured.

Background

Natural killer (NK) cells are members of the innate immune system. Their unique properties, including recognition of viral infected and tumor cells without major histocompatibility complex (MHC) restriction or prior sensitization, make them a suitable choice for immunotherapy. Low numbers of NK cells in circulating blood is the most important obstacle for this goal.

Umbilical Cord Blood;Natural Killer Cell;Mesenchymal Stem Cell Umbilical Cord Blood;Natural Killer Cell;Mesenchymal Stem Cell http://www.Biotech-health.com/index.php?page=article&article_id=33684 Mohammadhossein Ahmadi Mohammadhossein Ahmadi Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, IR Iran Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, IR Iran Ehteramolsadat Hosseini Ehteramolsadat Hosseini Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, IR Iran; Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Iranian Blood Transfusion Organization Building, P. O. Box: 146651157, Tehran, IR Iran. Tel: +98-9121599012, Fax: +98-2188628741 Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, IR Iran; Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Iranian Blood Transfusion Organization Building, P. O. Box: 146651157, Tehran, IR Iran. Tel: +98-9121599012, Fax: +98-2188628741 Ali Akbar Pourfathollah Ali Akbar Pourfathollah Professor of Immunology, Tarbiat Modares University, Tehran, IR Iran Professor of Immunology, Tarbiat Modares University, Tehran, IR Iran Mehran Ghasemzadeh Mehran Ghasemzadeh Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, IR Iran Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, IR Iran Gharib Karimi Gharib Karimi Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, IR Iran Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, IR Iran
en 10.17795/bhs-33334 Clinical Symptoms of Minor Head Trauma and Abnormal Computed Tomography Scan Clinical Symptoms of Minor Head Trauma and Abnormal Computed Tomography Scan research-article research-article Results

Loss of consciousness (GCS drop or amnesia) was markedly associated with abnormal brain CT scan (P < 0.05). Interestingly, we found 7 patients with normal clinical examination but significant abnormal brain CT scan.

Conclusions

According to the results of our study, we recommend that all patients with minor head trauma underwent brain CT scan in order not to miss any life-threatening head injuries.

Patients and Methods

We enrolled 680 patients presented to an academic trauma hospital with minor head trauma in a prospective manner. All participants underwent brain CT scan if they met the inclusion criteria and the results of scans were compared with clinical examination finding.

Background

Minor head trauma accounts for 70% to 90% of all head traumas. Previous studies stated that minor head traumas were associated with 7% - 20% significant abnormal findings in brain computed tomography (CT)-scans.

Objectives

The aim of this study was to reevaluate clinical criteria of taking brain CT scan in patients who suffered from minor head trauma.

Results

Loss of consciousness (GCS drop or amnesia) was markedly associated with abnormal brain CT scan (P < 0.05). Interestingly, we found 7 patients with normal clinical examination but significant abnormal brain CT scan.

Conclusions

According to the results of our study, we recommend that all patients with minor head trauma underwent brain CT scan in order not to miss any life-threatening head injuries.

Patients and Methods

We enrolled 680 patients presented to an academic trauma hospital with minor head trauma in a prospective manner. All participants underwent brain CT scan if they met the inclusion criteria and the results of scans were compared with clinical examination finding.

Background

Minor head trauma accounts for 70% to 90% of all head traumas. Previous studies stated that minor head traumas were associated with 7% - 20% significant abnormal findings in brain computed tomography (CT)-scans.

Objectives

The aim of this study was to reevaluate clinical criteria of taking brain CT scan in patients who suffered from minor head trauma.

Craniocerebral Trauma;Clinical Markers;Abnormalities;CT Scan Craniocerebral Trauma;Clinical Markers;Abnormalities;CT Scan http://www.Biotech-health.com/index.php?page=article&article_id=33334 Mohammadreza Maghsoudi Mohammadreza Maghsoudi Emergency Department, Tehran University of Medical Sciences, Tehran, IR Iran Emergency Department, Tehran University of Medical Sciences, Tehran, IR Iran Mehdi Samadzadeh Mehdi Samadzadeh Department of Psychiatry, Ardabil University of Medical Sciences, Ardabil, IR Iran Department of Psychiatry, Ardabil University of Medical Sciences, Ardabil, IR Iran Saghi Maghsoudi Saghi Maghsoudi Emergency Department, Hamadan University of Medical Sciences, Hamadan, IR Iran Emergency Department, Hamadan University of Medical Sciences, Hamadan, IR Iran Khatereh Isazadehfar Khatereh Isazadehfar Department of Community and Preventive Medicine, Ardabil University of Medical Sciences, Ardabil, IR Iran Department of Community and Preventive Medicine, Ardabil University of Medical Sciences, Ardabil, IR Iran Tooraj Asadi Tooraj Asadi Emergency Department, Tehran University of Medical Sciences, Tehran, IR Iran Emergency Department, Tehran University of Medical Sciences, Tehran, IR Iran Bita Shahbazzadegan Bita Shahbazzadegan Department of EDO , School of Medicine, Ardabil University of Medical Sciences, Ardabil, IR Iran; Department of Public Health, School of Health, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran; Department of Public Health, School of Health, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran. Tel: +914-4515848, Fax: +45-15510057 Department of EDO , School of Medicine, Ardabil University of Medical Sciences, Ardabil, IR Iran; Department of Public Health, School of Health, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran; Department of Public Health, School of Health, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran. Tel: +914-4515848, Fax: +45-15510057