Biotechnology and Health Sciences Biotechnology and Health Sciences Biotech Health Sci http://www.Biotech-health.com 2383-0271 2383-028X 10.5812/bhs en jalali 2017 5 28 gregorian 2017 5 28 2 3
en 10.17795/bhs-29562 Induction of Abortion in the First Trimester by Misoprostol or Misoprostol With Letrozole Induction of Abortion in the First Trimester by Misoprostol or Misoprostol With Letrozole research-article research-article Conclusions

Misoprostol plus letrozole was more effective for inducing abortion in the first trimester of pregnancy compared to misoprostol alone.

Results

The complete abortion rate was 69.7% in the misoprostol and letrozole group and 30.3% in the misoprostol group. Incomplete abortion was 32.4% in the misoprostol and letrozole group, and 67.6% in the misoprostol group (P = 0.004). Bleeding, cervix os opening time, and time to complete abortion from induction of drugs were similar in both groups (P > 0.05). There were no medical complications in both groups.

Patients and Methods

Seventy female candidates for legal abortion within the first trimester of pregnancy were divided to two groups: misoprostol alone and misoprostol with letrozole. The complete abortion, time to open the internal os of the cervix, time to complete the abortion, and drug-induced side effects of misoprostol and letrozole were recorded and analyzed.

Background

Management of abortion is an important issue in gynecology. Several millions of spontaneous abortions occur yearly and more than a million induced abortions are performed in the USA.

Objectives

The aim of this study was to compare the effect of misoprostol alone and misoprostol with letrozole in the induction of abortion in the first trimester of pregnancy in the Qazvin city of Iran.

Conclusions

Misoprostol plus letrozole was more effective for inducing abortion in the first trimester of pregnancy compared to misoprostol alone.

Results

The complete abortion rate was 69.7% in the misoprostol and letrozole group and 30.3% in the misoprostol group. Incomplete abortion was 32.4% in the misoprostol and letrozole group, and 67.6% in the misoprostol group (P = 0.004). Bleeding, cervix os opening time, and time to complete abortion from induction of drugs were similar in both groups (P > 0.05). There were no medical complications in both groups.

Patients and Methods

Seventy female candidates for legal abortion within the first trimester of pregnancy were divided to two groups: misoprostol alone and misoprostol with letrozole. The complete abortion, time to open the internal os of the cervix, time to complete the abortion, and drug-induced side effects of misoprostol and letrozole were recorded and analyzed.

Background

Management of abortion is an important issue in gynecology. Several millions of spontaneous abortions occur yearly and more than a million induced abortions are performed in the USA.

Objectives

The aim of this study was to compare the effect of misoprostol alone and misoprostol with letrozole in the induction of abortion in the first trimester of pregnancy in the Qazvin city of Iran.

Letrozole;Abortion;Induced;Misoprostol;Pregnancy Trimester, First Letrozole;Abortion;Induced;Misoprostol;Pregnancy Trimester, First http://www.Biotech-health.com/index.php?page=article&article_id=29562 Ezzatossadat Haj Seyed Javadi Ezzatossadat Haj Seyed Javadi Children Growth Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Children Growth Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Masoomeh Mohammadi Masoomeh Mohammadi Children Growth Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Children Growth Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Ameneh Barikani Ameneh Barikani Children Growth Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran; Children Growth Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel: +98-2833328709, Fax: +98-2833344088 Children Growth Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran; Children Growth Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel: +98-2833328709, Fax: +98-2833344088
en 10.17795/bhs-28234 Computer Vision Syndrome in Eleven to Eighteen-Year-Old Students in Qazvin Computer Vision Syndrome in Eleven to Eighteen-Year-Old Students in Qazvin research-article research-article Conclusions

Our findings revealed that using computers for prolonged periods of time can lead to eye-related problems such as eyestrain, headaches, weak and tired eyes, blurred and double vision, weak vision, burning itching and watery eyes, etc. To correct and reduce these problems the following precautions may be helpful, using computer shields (anti-reflex glasses), proper computer workplace illumination, enough rest after working with computers, appropriate viewing distances during computer work, placing computers far from bright objects and adjusting a line of vision with the top of the monitor.

Results

The results of this study indicated that 63.86% of the subjects had refractive errors. Refractive errors were significantly different in children of different genders (P < 0.05). The most common complaints associated with the continuous use of computers were eyestrain, eye pain, eye redness, headache, and blurred vision. The most prevalent (81.8%) eye-related problem in computer users was eyestrain and the least prevalent was dry eyes (7.84%). In order to reduce computer related problems 54.2% of the participants suggested taking enough rest, 37.9% recommended use of computers only for necessary tasks, while 24.4% and 19.1% suggested the use of monitor shields and proper working distance, respectively.

Background

Prolonged use of computers can lead to complications such as eye strain, eye and head aches, double and blurred vision, tired eyes, irritation, burning and itching eyes, eye redness, light sensitivity, dry eyes, muscle strains, and other problems.

Objectives

The aim of the present study was to evaluate visual problems and major symptoms, and their associations among computer users, aged between 11 and 18 years old, residing in the Qazvin city of Iran, during year 2010.

Patients and Methods

This cross-sectional study was done on 642 secondary to pre university students who had referred to the eye clinic of Buali hospital of Qazvin during year 2013. A questionnaire consisting of demographic information and 26 questions on visual effects of the computer was used to gather information. Participants answered all questions and then underwent complete eye examinations and in some cases cycloplegic refraction. Visual acuity (VA) was measured with a logMAR in six meters. Refraction errors were determined using an auto refractometer (Potece and Heine retinoscope). The collected data was then analyzed using the SPSS statistical software.

Conclusions

Our findings revealed that using computers for prolonged periods of time can lead to eye-related problems such as eyestrain, headaches, weak and tired eyes, blurred and double vision, weak vision, burning itching and watery eyes, etc. To correct and reduce these problems the following precautions may be helpful, using computer shields (anti-reflex glasses), proper computer workplace illumination, enough rest after working with computers, appropriate viewing distances during computer work, placing computers far from bright objects and adjusting a line of vision with the top of the monitor.

Results

The results of this study indicated that 63.86% of the subjects had refractive errors. Refractive errors were significantly different in children of different genders (P < 0.05). The most common complaints associated with the continuous use of computers were eyestrain, eye pain, eye redness, headache, and blurred vision. The most prevalent (81.8%) eye-related problem in computer users was eyestrain and the least prevalent was dry eyes (7.84%). In order to reduce computer related problems 54.2% of the participants suggested taking enough rest, 37.9% recommended use of computers only for necessary tasks, while 24.4% and 19.1% suggested the use of monitor shields and proper working distance, respectively.

Background

Prolonged use of computers can lead to complications such as eye strain, eye and head aches, double and blurred vision, tired eyes, irritation, burning and itching eyes, eye redness, light sensitivity, dry eyes, muscle strains, and other problems.

Objectives

The aim of the present study was to evaluate visual problems and major symptoms, and their associations among computer users, aged between 11 and 18 years old, residing in the Qazvin city of Iran, during year 2010.

Patients and Methods

This cross-sectional study was done on 642 secondary to pre university students who had referred to the eye clinic of Buali hospital of Qazvin during year 2013. A questionnaire consisting of demographic information and 26 questions on visual effects of the computer was used to gather information. Participants answered all questions and then underwent complete eye examinations and in some cases cycloplegic refraction. Visual acuity (VA) was measured with a logMAR in six meters. Refraction errors were determined using an auto refractometer (Potece and Heine retinoscope). The collected data was then analyzed using the SPSS statistical software.

Computer;Eye;Students Computer;Eye;Students http://www.Biotech-health.com/index.php?page=article&article_id=28234 Mohammad Khalaj Mohammad Khalaj Faculty of Health, Qazvin University of Medical Sciences, Qazvin, IR Iran; Faculty of Health, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel: +98-28333677285, Fax: +98-2833345862 Faculty of Health, Qazvin University of Medical Sciences, Qazvin, IR Iran; Faculty of Health, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel: +98-28333677285, Fax: +98-2833345862 Morteza Ebrahimi Morteza Ebrahimi Department of Medical Sciences, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Medical Sciences, Qazvin University of Medical Sciences, Qazvin, IR Iran Parisa Shojai Parisa Shojai Social Determinants of Health Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, IR Iran Social Determinants of Health Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, IR Iran Rafat Bagherzadeh Rafat Bagherzadeh English Department, School of Management and Health Information Services, Tehran University of Medical Sciences, Tehran, IR Iran English Department, School of Management and Health Information Services, Tehran University of Medical Sciences, Tehran, IR Iran Tahereh Sadeghi Tahereh Sadeghi Faculty of Nursing and Midwifery, Mashhad University of Medical Sciences, Mashhad, IR Iran Faculty of Nursing and Midwifery, Mashhad University of Medical Sciences, Mashhad, IR Iran Mehran Ghalenoei Mehran Ghalenoei Department of Occupational Health, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Occupational Health, Qazvin University of Medical Sciences, Qazvin, IR Iran
en 10.17795/bhs-30402 The Effect of Fenugreek (Trigonella foenum-graecum) Seed and 17-β Estradiol on Serum Apelin, Glucose, Lipids, and Insulin in Ovariectomized Rats The Effect of Fenugreek (<italic>Trigonella foenum-graecum</italic>) Seed and 17-β Estradiol on Serum Apelin, Glucose, Lipids, and Insulin in Ovariectomized Rats research-article research-article Conclusions

It appears that fenugreek seed might be effective against hyperglycemia, hyperlipidemia and insulin resistance in ovariectomized rats without impact on serum Apelin. Furthermore, 17-β estradiol could have similar effects along with possible inhibitory effects on serum Apelin. The complicated role of Apelin in menopause needs to be further explored.

Results

Serum Apelin, glucose, lipids and insulin significantly increased in ovariectomized controls in comparison with normal controls (P < 0.05). Serum glucose, lipids and insulin in ovariectomized rats treated with fenugreek seed extract and 17-β estradiol were remarkably lower than ovariectomized controls (P < 0.05). Furthermore, 17-β estradiol caused a significant decrease (P < 0.05) in serum Apelin in ovariectomized rats.

Materials and Methods

Forty-nine adult female Wistar rats were randomly divided to seven groups: normal control, ovariectomized control, ovariectomized treated with ethanolic and hexanic extract of fenugreek seed (50 and 150 mg/kg/daily for each), and ovariectomized treated with 17-β estradiol (10 µg/kg/daily) for 42 days. Serum Apelin, glucose, lipids and insulin were measured.

Background

Menopause, a natural phenomenon, is defined by the fall of ovarian hormones mainly estrogens causing major problems such as insulin resistance. Fenugreek (Trigonella foenum-graecum) is known to have some useful properties such as insulin sensitizing effect. Apelin is an adipokine, which has several roles such as regulation of insulin secretion.

Objectives

The objective of the present study was to evaluate the effect of fenugreek seed and 17-β estradiol on serum Apelin along with glucose, lipids and insulin in ovariectomized rats.

Conclusions

It appears that fenugreek seed might be effective against hyperglycemia, hyperlipidemia and insulin resistance in ovariectomized rats without impact on serum Apelin. Furthermore, 17-β estradiol could have similar effects along with possible inhibitory effects on serum Apelin. The complicated role of Apelin in menopause needs to be further explored.

Results

Serum Apelin, glucose, lipids and insulin significantly increased in ovariectomized controls in comparison with normal controls (P < 0.05). Serum glucose, lipids and insulin in ovariectomized rats treated with fenugreek seed extract and 17-β estradiol were remarkably lower than ovariectomized controls (P < 0.05). Furthermore, 17-β estradiol caused a significant decrease (P < 0.05) in serum Apelin in ovariectomized rats.

Materials and Methods

Forty-nine adult female Wistar rats were randomly divided to seven groups: normal control, ovariectomized control, ovariectomized treated with ethanolic and hexanic extract of fenugreek seed (50 and 150 mg/kg/daily for each), and ovariectomized treated with 17-β estradiol (10 µg/kg/daily) for 42 days. Serum Apelin, glucose, lipids and insulin were measured.

Background

Menopause, a natural phenomenon, is defined by the fall of ovarian hormones mainly estrogens causing major problems such as insulin resistance. Fenugreek (Trigonella foenum-graecum) is known to have some useful properties such as insulin sensitizing effect. Apelin is an adipokine, which has several roles such as regulation of insulin secretion.

Objectives

The objective of the present study was to evaluate the effect of fenugreek seed and 17-β estradiol on serum Apelin along with glucose, lipids and insulin in ovariectomized rats.

Apelin-13;Trigonella Foenum-Graecum;17 Beta-Estradiol;Ovariectomized;Insulin;Lipids Apelin-13;Trigonella Foenum-Graecum;17 Beta-Estradiol;Ovariectomized;Insulin;Lipids http://www.Biotech-health.com/index.php?page=article&article_id=30402 Mahmood Abedinzade Mahmood Abedinzade Medical Biotechnology Research Center, Department of Medical Physiology, Faculty of Paramedicine, Guilan University of Medical Sciences, Rasht, IR Iran Medical Biotechnology Research Center, Department of Medical Physiology, Faculty of Paramedicine, Guilan University of Medical Sciences, Rasht, IR Iran Sima Nasri Sima Nasri Department of Biology, Payame Noor University, Tehran, IR Iran Department of Biology, Payame Noor University, Tehran, IR Iran Masome Jamal Omidi Masome Jamal Omidi Department of Biology, Payame Noor University, Tehran, IR Iran Department of Biology, Payame Noor University, Tehran, IR Iran Bizhan Porramezan Bizhan Porramezan Department of Biology, Payame Noor University, Tehran, IR Iran Department of Biology, Payame Noor University, Tehran, IR Iran Korosh Khanaki Korosh Khanaki Medical Biotechnology Research Center, Department of Clinical Biochemistry, Faculty of Paramedicine, Guilan University of Medical Sciences, Rasht, IR Iran; Medical Biotechnology Research Center, Department of Clinical Biochemistry, Faculty of Paramedicine, Guilan University of Medical Sciences, Rasht, IR Iran. Tel: +98-1342536767, Fax: +98-1342537070 Medical Biotechnology Research Center, Department of Clinical Biochemistry, Faculty of Paramedicine, Guilan University of Medical Sciences, Rasht, IR Iran; Medical Biotechnology Research Center, Department of Clinical Biochemistry, Faculty of Paramedicine, Guilan University of Medical Sciences, Rasht, IR Iran. Tel: +98-1342536767, Fax: +98-1342537070
en 10.17795/bhs-27156 Immunization Status Against Hepatitis B Among Iranian Junior Medical, Nursing, and Obstetrics Students With Different Vaccination Patterns Immunization Status Against Hepatitis B Among Iranian Junior Medical, Nursing, and Obstetrics Students With Different Vaccination Patterns research-article research-article Conclusions

This study shows lower anti-HBs among students who were vaccinated at infancy compared to those vaccinated at older childhood or adolescence. Also, subsequent measurement of anti-HBs level at the time of entrance to university is recommended for all previously immunized students.

Results

Vaccination coverage rates were 74.1%. About half the participants didn’t show serological evidence of protective immunity; 68.9% had their last shot more than 10 years ago and 30.4% had a vaccination history of five years or less (P < 0.001). Geometric mean level of anti-HBs titer among students, who had received a primary series vaccine at birth, was significantly lower than students who had started vaccination at an older age (P < 0.001). Also, analysis of variance for geometric mean of anti-HBs titer showed significant differences between groups based on injection time from the last shot (P < 0.001) (post hoc comparisons resulted in a P value of < 0.001 for birth versus < 5 year group, and P < 0.001 for the 5 to 10 year group). The lowest rate of non-protective level belonged to participants with complete three doses and a booster additional shot (27.1%). The final model for independent predictors of anti-HBs positive status was made by a binary logistic regression analysis. The model included presence of a booster dose, injection time from last shot, and discipline of study.

Patients and Methods

In an analytical cross-sectional study, 255 junior medical sciences students were tested for quantitative antibodies to hepatitis B surface antigen (anti-HBs). The proportion of protective immunity was compared in different vaccination patterns.

Objectives

This study aimed to determine the status of immunity to hepatitis B in junior medical, nursing and obstetrics students with different hepatitis B virus (HBV) vaccination patterns.

Background

Since the protection time by hepatitis B (HB) vaccination is unclear, the strategy of immunization of junior students who previously received hepatitis vaccine is controversial.

Conclusions

This study shows lower anti-HBs among students who were vaccinated at infancy compared to those vaccinated at older childhood or adolescence. Also, subsequent measurement of anti-HBs level at the time of entrance to university is recommended for all previously immunized students.

Results

Vaccination coverage rates were 74.1%. About half the participants didn’t show serological evidence of protective immunity; 68.9% had their last shot more than 10 years ago and 30.4% had a vaccination history of five years or less (P < 0.001). Geometric mean level of anti-HBs titer among students, who had received a primary series vaccine at birth, was significantly lower than students who had started vaccination at an older age (P < 0.001). Also, analysis of variance for geometric mean of anti-HBs titer showed significant differences between groups based on injection time from the last shot (P < 0.001) (post hoc comparisons resulted in a P value of < 0.001 for birth versus < 5 year group, and P < 0.001 for the 5 to 10 year group). The lowest rate of non-protective level belonged to participants with complete three doses and a booster additional shot (27.1%). The final model for independent predictors of anti-HBs positive status was made by a binary logistic regression analysis. The model included presence of a booster dose, injection time from last shot, and discipline of study.

Patients and Methods

In an analytical cross-sectional study, 255 junior medical sciences students were tested for quantitative antibodies to hepatitis B surface antigen (anti-HBs). The proportion of protective immunity was compared in different vaccination patterns.

Objectives

This study aimed to determine the status of immunity to hepatitis B in junior medical, nursing and obstetrics students with different hepatitis B virus (HBV) vaccination patterns.

Background

Since the protection time by hepatitis B (HB) vaccination is unclear, the strategy of immunization of junior students who previously received hepatitis vaccine is controversial.

Hepatitis B;Vaccination;Medical Student;Antibody;Protective Immunity Hepatitis B;Vaccination;Medical Student;Antibody;Protective Immunity http://www.Biotech-health.com/index.php?page=article&article_id=27156 Abbas Allami Abbas Allami Department of Infectious Diseases, Faculty of Medicine, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Infectious Diseases, Faculty of Medicine, Qazvin University of Medical Sciences, Qazvin, IR Iran Navid Mohammadi Navid Mohammadi Department of Community Medicine, Faculty of Medicine, Iran University of Medical Sciences, Tehran, IR Iran; Department of Community Medicine, Faculty of Medicine, Iran University of Medical Sciences, Tehran, IR Iran. Tel: +98-2188602225 Department of Community Medicine, Faculty of Medicine, Iran University of Medical Sciences, Tehran, IR Iran; Department of Community Medicine, Faculty of Medicine, Iran University of Medical Sciences, Tehran, IR Iran. Tel: +98-2188602225 Azade Najar Azade Najar Department of Infectious Diseases, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Infectious Diseases, Qazvin University of Medical Sciences, Qazvin, IR Iran
en 10.17795/bhs-29887 Evaluation of N-Nitrosamine Formation in Routine Potato Cooking Evaluation of N-Nitrosamine Formation in Routine Potato Cooking research-article research-article Conclusions

Nitrosamine was formed with conventional potato baking methods with the most nitrosamine formation found on the surface fried samples. Nitrites amount in baked potatoes decreased. Generally, the amount of nitrosamine in baked potato samples was lower than acceptable limits.

Results

Fried samples that were measured by the gas chromatography method had the highest average levels of nitrosamine compounds; NDMA, 5.09 ng kg-1, and NDEA, 8.66 ng kg-1. Low levels of nitrosamine compounds were associated with raw potatoes, in which no nitrosamine compound was detected. Based on the analysis of the potato samples by spectrophotometry, the highest levels of nitrite was found in raw potatoes with a mean of 2.43 mg kg-1 and the lowest levels of nitrite were detected in boiled potatoes with an average of 1.172 mg kg-1.

Objectives

In this study we evaluated the effect of conventional potato cooking method on N-nitrosamine compound formation.

Materials and Methods

The amounts of four nitrosamines, namely N-nitrosodimethylamine (NDMA), N-nitrosodiethylamine (NDEA), N-nitrosopiperidine (NPIP) and N-nitrosopyrrolidine (NPYR) were determined in four different potato-baking methods. Sixty potato samples were randomly collected from Hamadan city. Fried potato samples were roasted at 180°C and boiled potato samples were scalded at 120°C. Nitrosamine levels were measured using gas chromatography coupled with electronic ionization detector (GC-EID), and spectrophotometry was used for measuring nitrite.

Background

Nitrosamine is amongst carcinogen chemical compounds, which can enter the human body through consumption of food. Potatoes are a root vegetable consumed by many people around the world, however their potential for nitrosamine formation during cooking processes needs to be considered for public health matters.

Conclusions

Nitrosamine was formed with conventional potato baking methods with the most nitrosamine formation found on the surface fried samples. Nitrites amount in baked potatoes decreased. Generally, the amount of nitrosamine in baked potato samples was lower than acceptable limits.

Results

Fried samples that were measured by the gas chromatography method had the highest average levels of nitrosamine compounds; NDMA, 5.09 ng kg-1, and NDEA, 8.66 ng kg-1. Low levels of nitrosamine compounds were associated with raw potatoes, in which no nitrosamine compound was detected. Based on the analysis of the potato samples by spectrophotometry, the highest levels of nitrite was found in raw potatoes with a mean of 2.43 mg kg-1 and the lowest levels of nitrite were detected in boiled potatoes with an average of 1.172 mg kg-1.

Objectives

In this study we evaluated the effect of conventional potato cooking method on N-nitrosamine compound formation.

Materials and Methods

The amounts of four nitrosamines, namely N-nitrosodimethylamine (NDMA), N-nitrosodiethylamine (NDEA), N-nitrosopiperidine (NPIP) and N-nitrosopyrrolidine (NPYR) were determined in four different potato-baking methods. Sixty potato samples were randomly collected from Hamadan city. Fried potato samples were roasted at 180°C and boiled potato samples were scalded at 120°C. Nitrosamine levels were measured using gas chromatography coupled with electronic ionization detector (GC-EID), and spectrophotometry was used for measuring nitrite.

Background

Nitrosamine is amongst carcinogen chemical compounds, which can enter the human body through consumption of food. Potatoes are a root vegetable consumed by many people around the world, however their potential for nitrosamine formation during cooking processes needs to be considered for public health matters.

Nitrosamines;Nitrites;Gas Chromatography Nitrosamines;Nitrites;Gas Chromatography http://www.Biotech-health.com/index.php?page=article&article_id=29887 Payman Qajarbeygi Payman Qajarbeygi Department of Health and Food Safety, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Health and Food Safety, Qazvin University of Medical Sciences, Qazvin, IR Iran Maryam Ahmadi Maryam Ahmadi Department of Health and Food Safety, Qazvin University of Medical Sciences, Qazvin, IR Iran; Department of Health and Food Safety, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel: +98-4116378743, Fax: +98-4116388743 Department of Health and Food Safety, Qazvin University of Medical Sciences, Qazvin, IR Iran; Department of Health and Food Safety, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel: +98-4116378743, Fax: +98-4116388743 Ashraf Haj Hoseini Ashraf Haj Hoseini Department of Food Science and Chemistry Thecnology, Danesh Mehvar Alborz Research Center, Qazvin, IR Iran Department of Food Science and Chemistry Thecnology, Danesh Mehvar Alborz Research Center, Qazvin, IR Iran Asghar Mohammad Poorasl Asghar Mohammad Poorasl Department of Health and Food Safety, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Health and Food Safety, Qazvin University of Medical Sciences, Qazvin, IR Iran Razzagh Mahmoudi Razzagh Mahmoudi Department of Food Hygiene and Aquatics, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, IR Iran Department of Food Hygiene and Aquatics, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, IR Iran Maryam Ataee Maryam Ataee Veterinary Medicine Faculty, Trehran University, Tehran, IR Iran Veterinary Medicine Faculty, Trehran University, Tehran, IR Iran
en 10.17795/bhs-28198 Effect of Caffeic Acid and Low-Power Laser Light Co-Exposure on Viability of Pseudomonas aeruginosa Effect of Caffeic Acid and Low-Power Laser Light Co-Exposure on Viability of <italic>Pseudomonas aeruginosa</italic> research-article research-article Conclusions

Caffeic acid treatment reduced bacterial growth and resulted in a decreased number of colony formation. The simultaneous effect of caffeic acid and laser at three time courses showed a synergic effect in reducing colony formation compared to the control and caffeic acid, and laser alone.

Objectives

The aim of this study was to investigate the effect of caffeic acid and low-power laser light co-exposure on Pseudomonas aeruginosa isolated from burn wounds.

Materials and Methods

Ten bacterial samples were collected from patients with burn wound infections at Shahid Motahhari medical center of Tehran. The He-Ne laser was used in this study with output power of 2 mW.

Results

The data significantly indicated that both the caffeic acid and laser treatment alone reduced the number of colony-forming units compared to control cultures. Co-exposure of bacterial suspensions to caffeic acid and laser at three time points showed the following number of colony-forming units 240.23 ± 60.15, 148.13 ± 52.66 and 84.57 ± 35, respectively. The best concentrations of caffeic acid to achieve countable colonies were 1.5 and 1.75 mM. At the concentration of 1.5 mM of caffeic acid, the number of colonies significantly reduced to 280.78 ± 59 (P = 0.008) while at 1.75 mM the number of colonies reduced to 234.07 ± 72.28 (P = 0.0001).

Background

The resistance of Pseudomonas aeruginosa to antibiotics is a big problem, especially in burns and wound infections. Laser irradiation affects microorganisms by denaturing their proteins, which involves changes in the chemical or physical properties of the protein.

Conclusions

Caffeic acid treatment reduced bacterial growth and resulted in a decreased number of colony formation. The simultaneous effect of caffeic acid and laser at three time courses showed a synergic effect in reducing colony formation compared to the control and caffeic acid, and laser alone.

Objectives

The aim of this study was to investigate the effect of caffeic acid and low-power laser light co-exposure on Pseudomonas aeruginosa isolated from burn wounds.

Materials and Methods

Ten bacterial samples were collected from patients with burn wound infections at Shahid Motahhari medical center of Tehran. The He-Ne laser was used in this study with output power of 2 mW.

Results

The data significantly indicated that both the caffeic acid and laser treatment alone reduced the number of colony-forming units compared to control cultures. Co-exposure of bacterial suspensions to caffeic acid and laser at three time points showed the following number of colony-forming units 240.23 ± 60.15, 148.13 ± 52.66 and 84.57 ± 35, respectively. The best concentrations of caffeic acid to achieve countable colonies were 1.5 and 1.75 mM. At the concentration of 1.5 mM of caffeic acid, the number of colonies significantly reduced to 280.78 ± 59 (P = 0.008) while at 1.75 mM the number of colonies reduced to 234.07 ± 72.28 (P = 0.0001).

Background

The resistance of Pseudomonas aeruginosa to antibiotics is a big problem, especially in burns and wound infections. Laser irradiation affects microorganisms by denaturing their proteins, which involves changes in the chemical or physical properties of the protein.

Caffeic Acid;Laser Therapy;Low-Level;Minimum Inhibitor Concentration;Pseudomonas aeruginosa Caffeic Acid;Laser Therapy;Low-Level;Minimum Inhibitor Concentration;Pseudomonas aeruginosa http://www.Biotech-health.com/index.php?page=article&article_id=28198 Nematolah Gheibi Nematolah Gheibi Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Nader Divan Khosroshahi Nader Divan Khosroshahi Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran; Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel: +98-9125819521, Fax: +98-2833324971 Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran; Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel: +98-9125819521, Fax: +98-2833324971 Marzieh Habibi Marzieh Habibi Microbiology Department, Science and Research Branch, Islamic Azad University, Tehran, IR Iran Microbiology Department, Science and Research Branch, Islamic Azad University, Tehran, IR Iran
en 10.17795/bhs30748 Evaluation of oprD Gene Expression in Carbapenem-Resistant Pseudomonas aeruginosa Strains Isolated From Severe Burn Patients With Secondary Infection Evaluation of <italic>oprD</italic> Gene Expression in Carbapenem-Resistant <italic>Pseudomonas aeruginosa</italic> Strains Isolated From Severe Burn Patients With Secondary Infection research-article research-article Conclusions

The results of this study indicated that a decrease in oprD gene expression is an important mechanism of resistance in carbapenem resistant Pseudomonas aeruginosa isolated from severe burn patients with secondary infection.

Results

Our study showed that 94.2% of the isolates were resistant to imipenem, 99.5% to meropenem, and all were resistant to ertapenem. The OprD gene expression among carbapenem resistant Pseudomonas aeruginosa isolates showed a 2 × 10-3 to 0.5 times decrease compared to the standard sensitive strain (P < 0.05).

Patients and Methods

One-hundred and eighty-nine clinical isolates of carbapenem resistant Pseudomonas aeruginosa, isolated from burn patients, were identified by microbiological methods followed by determination of antibiotic resistance pattern by the Kirby-Bauer procedure. The expression of oprD gene was determined by quantitative real-time polymerase chain reaction (PCR).

Objectives

This study was performed to investigate the prevalence of carbapenem-resistant strains of Pseudomonas aeruginosa in burn patients with secondary infection and also to evaluate oprD gene expression as a possible resistance mechanism to carbapenem in isolated carbapenem resistant P. aeruginosa strains.

Background

Pseudomonas aeruginosa is the most common pathogen isolated from severe burn patients with secondary infection. Since high resistance to most types of antibiotics is common among these bacteria, the treatment of infections caused by these agents is very difficult. Loss of oprD proteins from the outer membrane of bacterial cells causes a significant decrease in the sensitivity of Pseudomonas aeruginosa to carbapenems.

Conclusions

The results of this study indicated that a decrease in oprD gene expression is an important mechanism of resistance in carbapenem resistant Pseudomonas aeruginosa isolated from severe burn patients with secondary infection.

Results

Our study showed that 94.2% of the isolates were resistant to imipenem, 99.5% to meropenem, and all were resistant to ertapenem. The OprD gene expression among carbapenem resistant Pseudomonas aeruginosa isolates showed a 2 × 10-3 to 0.5 times decrease compared to the standard sensitive strain (P < 0.05).

Patients and Methods

One-hundred and eighty-nine clinical isolates of carbapenem resistant Pseudomonas aeruginosa, isolated from burn patients, were identified by microbiological methods followed by determination of antibiotic resistance pattern by the Kirby-Bauer procedure. The expression of oprD gene was determined by quantitative real-time polymerase chain reaction (PCR).

Objectives

This study was performed to investigate the prevalence of carbapenem-resistant strains of Pseudomonas aeruginosa in burn patients with secondary infection and also to evaluate oprD gene expression as a possible resistance mechanism to carbapenem in isolated carbapenem resistant P. aeruginosa strains.

Background

Pseudomonas aeruginosa is the most common pathogen isolated from severe burn patients with secondary infection. Since high resistance to most types of antibiotics is common among these bacteria, the treatment of infections caused by these agents is very difficult. Loss of oprD proteins from the outer membrane of bacterial cells causes a significant decrease in the sensitivity of Pseudomonas aeruginosa to carbapenems.

Drug Resistance;OprD;Pseudomonas aeruginosa Drug Resistance;OprD;Pseudomonas aeruginosa http://www.Biotech-health.com/index.php?page=article&article_id=30748 Akram Azimi Akram Azimi Department of Microbiology, Medical School, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Microbiology, Medical School, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Taghi Naserpour Taghi Naserpour Department of Microbiology, Medical School, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran; Department of Microbiology, Medical School, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel: +98-91288014010; Fax: +98-2813324971 Department of Microbiology, Medical School, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran; Department of Microbiology, Medical School, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel: +98-91288014010; Fax: +98-2813324971 Fariba Bazmi Fariba Bazmi Department of Microbiology, Medical School, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Microbiology, Medical School, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Amir Peymani Amir Peymani Department of Microbiology, Medical School, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Microbiology, Medical School, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Masumeh Aslanimehr Masumeh Aslanimehr Department of Microbiology, Medical School, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Microbiology, Medical School, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Saman Saadat Saman Saadat Department of Microbiology, Medical School, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Microbiology, Medical School, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran
en 10.17795/bhs30256 Behavioral Modification of Parents With the Barkley Method to Increase Resilience of Mothers of Children With Attention Deficit/Hyperactivity Disorder Behavioral Modification of Parents With the Barkley Method to Increase Resilience of Mothers of Children With Attention Deficit/Hyperactivity Disorder research-article research-article Results

The behavioral modification of mothers with the Barkley method significantly enhanced their resiliency with children of ADHD in the experimental group compared to the control group.

Patients and Methods

Twenty-four mothers of children diagnosed with ADHD were randomly enrolled in this study. The mothers were selected from the 22nd of Bahman hospital of Qazvin city. Mothers with a low score in resilience with children aged between four and twelve years were assigned to experimental and control groups. In this study, a questionnaire was used to measure resilience. The data were analyzed by covariance analysis.

Objectives

To determine the efficacy of behavioral modification with Barkley method on resilience of mothers of children with attention deficit/hyperactivity disorder (ADHD).

Background

Mothers of children with attention deficit/hyperactivity disorder are faced with more stress than mothers of healthy children and this extreme stress can affect their ability to train their children effectively. Psychologists believe that there is a moderating factor between stressful events and psychological disorders that cause stressful events to have different effects on individuals. A type of this moderating factor is resiliency.

Conclusions

The behavioral modification was found to be effective for the resilience of mothers of children with ADHD.

Results

The behavioral modification of mothers with the Barkley method significantly enhanced their resiliency with children of ADHD in the experimental group compared to the control group.

Patients and Methods

Twenty-four mothers of children diagnosed with ADHD were randomly enrolled in this study. The mothers were selected from the 22nd of Bahman hospital of Qazvin city. Mothers with a low score in resilience with children aged between four and twelve years were assigned to experimental and control groups. In this study, a questionnaire was used to measure resilience. The data were analyzed by covariance analysis.

Objectives

To determine the efficacy of behavioral modification with Barkley method on resilience of mothers of children with attention deficit/hyperactivity disorder (ADHD).

Background

Mothers of children with attention deficit/hyperactivity disorder are faced with more stress than mothers of healthy children and this extreme stress can affect their ability to train their children effectively. Psychologists believe that there is a moderating factor between stressful events and psychological disorders that cause stressful events to have different effects on individuals. A type of this moderating factor is resiliency.

Conclusions

The behavioral modification was found to be effective for the resilience of mothers of children with ADHD.

Attention Deficit/Hyperactivity Disorder (ADHD);Resilience;Behavioral Modification Attention Deficit/Hyperactivity Disorder (ADHD);Resilience;Behavioral Modification http://www.Biotech-health.com/index.php?page=article&article_id=30256 Seyed Alireza Haji Seyed Javadi Seyed Alireza Haji Seyed Javadi Department of Psychiatry, 22nd of Bahman Hospital, Qazvin University of Medical Sciences, Qazvin, IR Iran; Department of Psychiatry, 22nd of Bahman Hospital, Qazvin university of Medical Sciences, Qazvin, IR Iran. Tel: +98-2833565712, Fax: +98-2833555054 Department of Psychiatry, 22nd of Bahman Hospital, Qazvin University of Medical Sciences, Qazvin, IR Iran; Department of Psychiatry, 22nd of Bahman Hospital, Qazvin university of Medical Sciences, Qazvin, IR Iran. Tel: +98-2833565712, Fax: +98-2833555054 Zahra Soltani Pari Zahra Soltani Pari Department of Personality Psychology, Islamic Azad University, Karaj, IR Iran Department of Personality Psychology, Islamic Azad University, Karaj, IR Iran Tahere Haji Seyed Javadi Tahere Haji Seyed Javadi Department of Health Psychology, Islamic Azad University, Karaj, IR Iran Department of Health Psychology, Islamic Azad University, Karaj, IR Iran
en 10.17795/bhs27414 The Relationship Between IMP3 Expression in Colorectal Adenocarcinoma and Clinicopathologic Findings The Relationship Between IMP3 Expression in Colorectal Adenocarcinoma and Clinicopathologic Findings research-article research-article Conclusions

IMP3 immunoreactivity was associated with poor differentiation of tumor yet not associated with tumor size depth of invasion or lymph node involvement.

Results

Immunoreactivity pattern of IMP3 was cytoplasmic in different clinicopathologic findings. Among different clinicopathologic findings, we found a statistical relationship between tumor differentiation and IMP3 (P = 0.047); so that, the poorly differentiated tumors were positive for this marker. No relationship was found between tumor size, depth of invasion or lymph node involvement and IMP3.

Objectives

In this study, the relationship between IMP3 expression in colorectal adenocarcinoma and clinicopathologic findings was assessed.

Patients and Methods

In this study 112 colorectal tumor paraffin blocks of Rasoul-e-Akram hospital were stained for IMP3 and slides were assessed for intensity and extent of positivity. The statistical relationships between marker expression and clinicopathologic findings (degree of differentiation, tumor size, depth of invasion and lymph nodes metastasis) were assessed. Data were analyzed by the SPSS 21 software and logistic regression and chi-square test, with p-values of less than 0.05 indicating statistical significance.

Background

The IMP3 is an oncofetal protein, which has been recently proposed as a diagnostic and prognostic marker in many cancers, including colorectal adenocarcinoma. The overexpression of IMP3 seems to have a correlation with patient’s prognosis.

Conclusions

IMP3 immunoreactivity was associated with poor differentiation of tumor yet not associated with tumor size depth of invasion or lymph node involvement.

Results

Immunoreactivity pattern of IMP3 was cytoplasmic in different clinicopathologic findings. Among different clinicopathologic findings, we found a statistical relationship between tumor differentiation and IMP3 (P = 0.047); so that, the poorly differentiated tumors were positive for this marker. No relationship was found between tumor size, depth of invasion or lymph node involvement and IMP3.

Objectives

In this study, the relationship between IMP3 expression in colorectal adenocarcinoma and clinicopathologic findings was assessed.

Patients and Methods

In this study 112 colorectal tumor paraffin blocks of Rasoul-e-Akram hospital were stained for IMP3 and slides were assessed for intensity and extent of positivity. The statistical relationships between marker expression and clinicopathologic findings (degree of differentiation, tumor size, depth of invasion and lymph nodes metastasis) were assessed. Data were analyzed by the SPSS 21 software and logistic regression and chi-square test, with p-values of less than 0.05 indicating statistical significance.

Background

The IMP3 is an oncofetal protein, which has been recently proposed as a diagnostic and prognostic marker in many cancers, including colorectal adenocarcinoma. The overexpression of IMP3 seems to have a correlation with patient’s prognosis.

IMP3 Protein;Human;Colorectal Cancer;Clinicopathologic Finding IMP3 Protein;Human;Colorectal Cancer;Clinicopathologic Finding http://www.Biotech-health.com/index.php?page=article&article_id=27414 Fatemeh Radfar Fatemeh Radfar Department of Anatomy, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Anatomy, Qazvin University of Medical Sciences, Qazvin, IR Iran Farzad Achak Farzad Achak Department of Pathology, Alborz University of Medical Sciences, Karaj, IR Iran Department of Pathology, Alborz University of Medical Sciences, Karaj, IR Iran Farzad Rajaei Farzad Rajaei Department of Anatomy, Qazvin University of Medical Sciences, Qazvin, IR Iran; Cellular and Molecular Research Centre, Qazvin University of Medical Sciences, Qazvin, IR Iran; Cellular and Molecular Research Centre, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel: +98-9122817421, Fax: +98-2813324970 Department of Anatomy, Qazvin University of Medical Sciences, Qazvin, IR Iran; Cellular and Molecular Research Centre, Qazvin University of Medical Sciences, Qazvin, IR Iran; Cellular and Molecular Research Centre, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel: +98-9122817421, Fax: +98-2813324970