Biotechnology and Health Sciences Biotechnology and Health Sciences Biotech Health Sci http://www.Biotech-health.com 2383-0271 2383-028X 10.5812/bhs en jalali 2017 5 28 gregorian 2017 5 28 1 1
en 10.17795/bhs-19188 Traditional Practices Affecting Maternal Care in Rural Areas of Qazvin: A Qualitative Study Traditional Practices Affecting Maternal Care in Rural Areas of Qazvin: A Qualitative Study research-article research-article Background

Tradition is a part of people’s culture that can influence the health care. It is, therefore, necessary to identify the habits that change the health behavior of people.

Objectives

In this study, we aimed to spot the traditions affecting maternity cares in the rural areas of Qazvin province, Iran.

Patients and Methods

A qualitative approach (ethnographic research) was applied. Data were collected through observation, group interviews and note-taking within seven villages with about 2500 households. The data were coded and classified and presented.

Results

The caring traditions were classified into such groups as during labor, bearing and postpartum, nutrition of the mother, caring of umbilical cord, curative care of icteric newborn, the mother’s and newborn’s nursing and garments, and caring of breast aches and inflammations. Some of traditional behaviors are useful such as washing the newborn and mental support of the motherhood. And some are harmful such as laying the laboring women on the cow dung etc, where some of the other ethnic habits may have unknown effects whether good or bad.

Conclusions

Subcultures can influence the health care behavior of the people and they should be identified and analyzed for the “change” of the health related behaviors.

Background

Tradition is a part of people’s culture that can influence the health care. It is, therefore, necessary to identify the habits that change the health behavior of people.

Objectives

In this study, we aimed to spot the traditions affecting maternity cares in the rural areas of Qazvin province, Iran.

Patients and Methods

A qualitative approach (ethnographic research) was applied. Data were collected through observation, group interviews and note-taking within seven villages with about 2500 households. The data were coded and classified and presented.

Results

The caring traditions were classified into such groups as during labor, bearing and postpartum, nutrition of the mother, caring of umbilical cord, curative care of icteric newborn, the mother’s and newborn’s nursing and garments, and caring of breast aches and inflammations. Some of traditional behaviors are useful such as washing the newborn and mental support of the motherhood. And some are harmful such as laying the laboring women on the cow dung etc, where some of the other ethnic habits may have unknown effects whether good or bad.

Conclusions

Subcultures can influence the health care behavior of the people and they should be identified and analyzed for the “change” of the health related behaviors.

Culture;Maternity;Newborn;Health Care Culture;Maternity;Newborn;Health Care http://www.Biotech-health.com/index.php?page=article&article_id=19188 Saeed Asefzadeh Saeed Asefzadeh Social Determinants of Health Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran; Social Determinants of Health Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel: +98-2813337006, Fax: +98-2813350056 Social Determinants of Health Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran; Social Determinants of Health Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel: +98-2813337006, Fax: +98-2813350056 Fariba Taherkhani Fariba Taherkhani The Health Network, Qazvin University of Medical Sciences, Qazvin, IR Iran The Health Network, Qazvin University of Medical Sciences, Qazvin, IR Iran Ahmad Ghodosian Ahmad Ghodosian The Health Network, Qazvin University of Medical Sciences, Qazvin, IR Iran The Health Network, Qazvin University of Medical Sciences, Qazvin, IR Iran
en 10.17795/whb.19189 Comparing the Severity of Coronary Artery Stenosis in Diabetic and Nondiabetic Patients Comparing the Severity of Coronary Artery Stenosis in Diabetic and Nondiabetic Patients research-article research-article Conclusions

Our findings revealed that the severity of coronary artery stenosis is common in diabetic patients compared to nondiabetics. Early diagnosis and treatment of CAD in diabetic patients is recommended.

Results

Based on data found in our study, 93.8% of diabetic and 83.4% of nondiabetic patients were shown to suffer coronary artery stenosis. Severe involvement (grade 3VD) of coronary artery stenosis was observed in 44.6% and 28.8% of diabetic and nondiabetic patients, respectively. Statistically, a significant difference was found between two groups regarding the rate and severity of CAD (P < 0.05). The occurrence of coronary stenosis was higher in females among both groups yet the difference insignificant, statistically (P > 0.05).

Objectives

The aim of present study was to compare the extent of CAD in the diabetic and nondiabetic patients.

Patients and Methods

In this case-control study 65 diabetic patients (case group) were compared with 145 nondiabetic patients (control group) based on severity of coronary artery stenosis at heart center of Mostafa Khomeini hospital in Tehran (Iran) in 2007. Both groups were matched for age, sex and risk factors. Coronary artery status was evaluated by coronary angiography followed by analysis of data using statistical methods.

Background

Diabetes is a common disease. There are some reports that indicate higher prevalence of coronary artery disease (CAD) in diabetic than nondiabetic patients, thus evaluating CAD in such patients is of prime importance.

Conclusions

Our findings revealed that the severity of coronary artery stenosis is common in diabetic patients compared to nondiabetics. Early diagnosis and treatment of CAD in diabetic patients is recommended.

Results

Based on data found in our study, 93.8% of diabetic and 83.4% of nondiabetic patients were shown to suffer coronary artery stenosis. Severe involvement (grade 3VD) of coronary artery stenosis was observed in 44.6% and 28.8% of diabetic and nondiabetic patients, respectively. Statistically, a significant difference was found between two groups regarding the rate and severity of CAD (P < 0.05). The occurrence of coronary stenosis was higher in females among both groups yet the difference insignificant, statistically (P > 0.05).

Objectives

The aim of present study was to compare the extent of CAD in the diabetic and nondiabetic patients.

Patients and Methods

In this case-control study 65 diabetic patients (case group) were compared with 145 nondiabetic patients (control group) based on severity of coronary artery stenosis at heart center of Mostafa Khomeini hospital in Tehran (Iran) in 2007. Both groups were matched for age, sex and risk factors. Coronary artery status was evaluated by coronary angiography followed by analysis of data using statistical methods.

Background

Diabetes is a common disease. There are some reports that indicate higher prevalence of coronary artery disease (CAD) in diabetic than nondiabetic patients, thus evaluating CAD in such patients is of prime importance.

Diabetes Mellitus;Coronary Artery Disease;Coronary Stenosis;Angiography Diabetes Mellitus;Coronary Artery Disease;Coronary Stenosis;Angiography http://www.Biotech-health.com/index.php?page=article&article_id=19189 Morteza Ebrahimi Morteza Ebrahimi Department of Cardiology, Boali Hospital, Qazvin University of Medical Sciences, Qazvin, IR Iran; Department of Cardiology, Boali Hospital, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel: +98-2813665473, Fax: +98-2813326033 Department of Cardiology, Boali Hospital, Qazvin University of Medical Sciences, Qazvin, IR Iran; Department of Cardiology, Boali Hospital, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel: +98-2813665473, Fax: +98-2813326033 Ali Asghar Pahlavan Ali Asghar Pahlavan Department of Microbiology, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Microbiology, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Shima Salimi Shima Salimi Department of Cardiology, Boali Hospital, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Cardiology, Boali Hospital, Qazvin University of Medical Sciences, Qazvin, IR Iran Mohammad Khalaj Mohammad Khalaj Department of Health Sciences, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Health Sciences, Qazvin University of Medical Sciences, Qazvin, IR Iran
en 10.17795/bhs-19190 Association Between the Serum Levels of Zinc, Copper and Lipid Profile With Osteoporosis in Iranian Postmenopausal Women Association Between the Serum Levels of Zinc, Copper and Lipid Profile With Osteoporosis in Iranian Postmenopausal Women research-article research-article Background

Trace elements and lipids have an important role in the development of osteoporosis that is a major health problem of postmenopausal women.

Objectives

The purpose of this study was to compare the serum levels of zinc (Zn), copper (Cu) and lipid profile between the postmenopausal women suffering from osteoporosis and the healthy controls. Furthermore, we aimed to determine whether there is an association between the parameters mentioned above and the bone mineral density (BMD).

Patients and Methods

The study was conducted on 116 postmenopausal women; 58 osteoporosis (age 60.6 ± 3.9 years) and 58 control group (age 55.4 ± 1.7 years). The serum levels of Zn and Cu were measured by atomic absorption spectrophotometry and BMD was analyzed by DEXA scan.

Results

The serum levels of Zn and Cu were similar in the both groups (P > 0.05). Serum levels of low density lipoprotein (LDL) and total cholesterol (TC) in osteoporosis group was statistically significant when compared to the controls (P < 0.05). Correlation analysis showed that there was significant association between body mass index (BMI) and BMD values (P < 0.05). There was no correlation between serum Zn, Cu levels with lipid profile (P > 0.05). However, we found a negative significant correlation between BMD with LDL (r = -0.31, P = 0.001) and total cholesterol levels (r = -0.26, P = 0.006).

Conclusions

This study suggested that dyslipidemia might be an independent risk factor of osteoporosis in Iranian postmenopausal women. Moreover, the trace elements did not directly and correlatively influence BMD.

Background

Trace elements and lipids have an important role in the development of osteoporosis that is a major health problem of postmenopausal women.

Objectives

The purpose of this study was to compare the serum levels of zinc (Zn), copper (Cu) and lipid profile between the postmenopausal women suffering from osteoporosis and the healthy controls. Furthermore, we aimed to determine whether there is an association between the parameters mentioned above and the bone mineral density (BMD).

Patients and Methods

The study was conducted on 116 postmenopausal women; 58 osteoporosis (age 60.6 ± 3.9 years) and 58 control group (age 55.4 ± 1.7 years). The serum levels of Zn and Cu were measured by atomic absorption spectrophotometry and BMD was analyzed by DEXA scan.

Results

The serum levels of Zn and Cu were similar in the both groups (P > 0.05). Serum levels of low density lipoprotein (LDL) and total cholesterol (TC) in osteoporosis group was statistically significant when compared to the controls (P < 0.05). Correlation analysis showed that there was significant association between body mass index (BMI) and BMD values (P < 0.05). There was no correlation between serum Zn, Cu levels with lipid profile (P > 0.05). However, we found a negative significant correlation between BMD with LDL (r = -0.31, P = 0.001) and total cholesterol levels (r = -0.26, P = 0.006).

Conclusions

This study suggested that dyslipidemia might be an independent risk factor of osteoporosis in Iranian postmenopausal women. Moreover, the trace elements did not directly and correlatively influence BMD.

Osteoporosis;Postmenopause;Bone Density;Lipids;Zinc;Copper Osteoporosis;Postmenopause;Bone Density;Lipids;Zinc;Copper http://www.Biotech-health.com/index.php?page=article&article_id=19190 Mehdi Sahmani Mehdi Sahmani Department of Clinical Biochemistry, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Clinical Biochemistry, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Shideh Omidian Shideh Omidian Department of Internal Medicine, Metabolism and Endocrine Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Internal Medicine, Metabolism and Endocrine Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Amir Javadi Amir Javadi Department of Social Medicine, Faculty of Medicine, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Social Medicine, Faculty of Medicine, Qazvin University of Medical Sciences, Qazvin, IR Iran Majid Sirati Sabet Majid Sirati Sabet Department of Clinical Biochemistry, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Clinical Biochemistry, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Mahnaz Abbasi Mahnaz Abbasi Department of Internal Medicine, Metabolism and Endocrine Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran; Department of Internal Medicine, Metabolism and Endocrine Search Center, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel: +98-2813328212, Fax: +98-2813328213 Department of Internal Medicine, Metabolism and Endocrine Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran; Department of Internal Medicine, Metabolism and Endocrine Search Center, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel: +98-2813328212, Fax: +98-2813328213
en 10.17795/bhs-19191 Cloning and Expression of Mycobacterium Tuberculosis Rv0285 (Pe5) Gene Cloning and Expression of Mycobacterium Tuberculosis Rv0285 (Pe5) Gene research-article research-article Background

PE5 is a member of the PE protein family whose precise function is yet to be understood. There are about 100 members of the PE family proteins in mycobacterium tuberculosis. These glycine- and alanine-rich proteins consist of Proline-Glutamate motifs at their N-termini and may be implicated in pathogenesis of the bacilli.

Objectives

We aimed to clone and over-express the Rv0285 coding region in the BL21 (DE3) Escherichia coli strain for the future functional investigations.

Materials and Methods

The PE5 coding region was cloned into a specific vector containing N-terminal GST tag using ligation independent cloning (LIC) method and then the recombinant vector was transferred into the competent TOP10 E. coli strain. The positive colonies were screened by the colony PCR approach and finally integration of the constructed expression vector was assessed by DNA sequencing. The vectors were then transferred and expressed in E. coli BL21 (DE3) strain and finally the protein expression level was analyzed using SDS-PAGE.

Results

In this study, the PE5 (Rv0285) coding region was amplified as a 309 bp DNA fragment from the mycobacterium tuberculosis H37Rv chromosome using specific sets of primers. The amplicon was then cloned into pLEICS-02 and then confirmed by DNA sequencing. The recombinant protein was over-expressed as a ~ 40 kDa tagged protein in E. coli, and finally confirmed by SDS-PAGE analysis.

Conclusions

Our data showed that recombinant PE5 coding region was successfully cloned in pLEICS-02 and expressed in BL21 (DE3) E. coli strain as host.

Background

PE5 is a member of the PE protein family whose precise function is yet to be understood. There are about 100 members of the PE family proteins in mycobacterium tuberculosis. These glycine- and alanine-rich proteins consist of Proline-Glutamate motifs at their N-termini and may be implicated in pathogenesis of the bacilli.

Objectives

We aimed to clone and over-express the Rv0285 coding region in the BL21 (DE3) Escherichia coli strain for the future functional investigations.

Materials and Methods

The PE5 coding region was cloned into a specific vector containing N-terminal GST tag using ligation independent cloning (LIC) method and then the recombinant vector was transferred into the competent TOP10 E. coli strain. The positive colonies were screened by the colony PCR approach and finally integration of the constructed expression vector was assessed by DNA sequencing. The vectors were then transferred and expressed in E. coli BL21 (DE3) strain and finally the protein expression level was analyzed using SDS-PAGE.

Results

In this study, the PE5 (Rv0285) coding region was amplified as a 309 bp DNA fragment from the mycobacterium tuberculosis H37Rv chromosome using specific sets of primers. The amplicon was then cloned into pLEICS-02 and then confirmed by DNA sequencing. The recombinant protein was over-expressed as a ~ 40 kDa tagged protein in E. coli, and finally confirmed by SDS-PAGE analysis.

Conclusions

Our data showed that recombinant PE5 coding region was successfully cloned in pLEICS-02 and expressed in BL21 (DE3) E. coli strain as host.

Mycobacterium Tuberculosis;Genes Mycobacterium Tuberculosis;Genes http://www.Biotech-health.com/index.php?page=article&article_id=19191 Majid Sirati-Sabet Majid Sirati-Sabet Department of Biochemistry and Genetic, Qazvin University of Medical Sciences, Qazvin, IR Iran; Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Biochemistry and Genetic, Qazvin University of Medical Sciences, Qazvin, IR Iran; Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Mehdi Sahmani Mehdi Sahmani Department of Biochemistry and Genetic, Qazvin University of Medical Sciences, Qazvin, IR Iran; Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Biochemistry and Genetic, Qazvin University of Medical Sciences, Qazvin, IR Iran; Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Reza Najafipour Reza Najafipour Department of Biochemistry and Genetic, Qazvin University of Medical Sciences, Qazvin, IR Iran; Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Biochemistry and Genetic, Qazvin University of Medical Sciences, Qazvin, IR Iran; Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Safarali Alizadeh Safarali Alizadeh Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Hossein Piri Hossein Piri Department of Biochemistry and Genetic, Qazvin University of Medical Sciences, Qazvin, IR Iran; Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Biochemistry and Genetic, Qazvin University of Medical Sciences, Qazvin, IR Iran; Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Zohreh Abdolvahabi Zohreh Abdolvahabi Department of Biochemistry and Genetic, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Biochemistry and Genetic, Qazvin University of Medical Sciences, Qazvin, IR Iran Farhad Khabbaz Farhad Khabbaz Department of Biochemistry and Genetic, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Biochemistry and Genetic, Qazvin University of Medical Sciences, Qazvin, IR Iran Dariush Ilghari Dariush Ilghari Department of Biochemistry and Genetic, Qazvin University of Medical Sciences, Qazvin, IR Iran; Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran; Department of Clinical Biochemistry and Genetics, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel: +98-9382081127 Department of Biochemistry and Genetic, Qazvin University of Medical Sciences, Qazvin, IR Iran; Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran; Department of Clinical Biochemistry and Genetics, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel: +98-9382081127
en 10.17795/bhs-19192 The Effect of Lead on Erythrocyte Glucose-6-Phosphate Dehydrogenase Activity in Rats The Effect of Lead on Erythrocyte Glucose-6-Phosphate Dehydrogenase Activity in Rats research-article research-article Conclusions

The results of this study showed that exposure to lead increased the activity of G6PD in the rat erythrocytes, perhaps resulting in an up regulation of the enzyme to detoxify lead.

Materials and Methods

In this study 14 albino rats were divided into two groups of seven animals. The treated group was exposed to 2% lead acetate in the drinking water during eight weeks. The control group was kept in the similar condition as the test group; however this group was not exposed to lead acetate. The blood lead level was measured with an atomic absorption spectrophotometer. The G6PD activity was determined by kinetic method. The hemoglobin content was determined by Drabkin's method. Malondialdehyde (MDA) in rats' plasma was measured with the thiobarbituric acid test using HPLC.

Background

Exposure to lead damages the biological systems, developing oxidative stress. Glucose-6-phosphate dehydrogenase (G6PD) produces NADPH in pentose phosphate pathway. This molecule protects tissues from oxidative stress. There are conflicting reports in the literatures of the effects of lead on G6PD activity.

Results

The blood lead concentration in treated group was increased when compared to control group (P < 0.05). A significant decrease in hemoglobin level was noted in lead-treated animals (P < 0.05). The G6PD activity in erythrocytes of rats received lead acetate increased up to 81% compared to the control group (P < 0.05). The G6PD/hemoglobin ratio in control and treatment groups was 17.7 ± 3.6 U/g and 44.9 ± 4.4 U/g, respectively. A significant increase in the plasma MDA level was also observed in the exposed group (P < 0.05).

Objectives

The aim of this investigation was to evaluate the effect of lead on erythrocyte G6PD activity in rats given lead acetate in their drinking water.

Conclusions

The results of this study showed that exposure to lead increased the activity of G6PD in the rat erythrocytes, perhaps resulting in an up regulation of the enzyme to detoxify lead.

Materials and Methods

In this study 14 albino rats were divided into two groups of seven animals. The treated group was exposed to 2% lead acetate in the drinking water during eight weeks. The control group was kept in the similar condition as the test group; however this group was not exposed to lead acetate. The blood lead level was measured with an atomic absorption spectrophotometer. The G6PD activity was determined by kinetic method. The hemoglobin content was determined by Drabkin's method. Malondialdehyde (MDA) in rats' plasma was measured with the thiobarbituric acid test using HPLC.

Background

Exposure to lead damages the biological systems, developing oxidative stress. Glucose-6-phosphate dehydrogenase (G6PD) produces NADPH in pentose phosphate pathway. This molecule protects tissues from oxidative stress. There are conflicting reports in the literatures of the effects of lead on G6PD activity.

Results

The blood lead concentration in treated group was increased when compared to control group (P < 0.05). A significant decrease in hemoglobin level was noted in lead-treated animals (P < 0.05). The G6PD activity in erythrocytes of rats received lead acetate increased up to 81% compared to the control group (P < 0.05). The G6PD/hemoglobin ratio in control and treatment groups was 17.7 ± 3.6 U/g and 44.9 ± 4.4 U/g, respectively. A significant increase in the plasma MDA level was also observed in the exposed group (P < 0.05).

Objectives

The aim of this investigation was to evaluate the effect of lead on erythrocyte G6PD activity in rats given lead acetate in their drinking water.

Glucose-6-Phosphate Dehydrogenase;Malondialdehyde;Lead;Oxidative Stress;Rats Glucose-6-Phosphate Dehydrogenase;Malondialdehyde;Lead;Oxidative Stress;Rats http://www.Biotech-health.com/index.php?page=article&article_id=19192 Majid Sirati-Sabet Majid Sirati-Sabet Department of Clinical Biochemistry and Genetics, Qazvin University of Medical Sciences, Qazvin, IR Iran; Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Clinical Biochemistry and Genetics, Qazvin University of Medical Sciences, Qazvin, IR Iran; Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Gholamreza Asadikaram Gholamreza Asadikaram Department of Biochemistry, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, IR Iran; Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, IR Iran; Department of Biochemistry, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, IR Iran. Tel: +98-9131406916, Fax: +98-3413222048 Department of Biochemistry, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, IR Iran; Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, IR Iran; Department of Biochemistry, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, IR Iran. Tel: +98-9131406916, Fax: +98-3413222048 Ali Safari-Varyani Ali Safari-Varyani Department of Occupational Health, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Occupational Health, Qazvin University of Medical Sciences, Qazvin, IR Iran Dariush Ilghari Dariush Ilghari Department of Clinical Biochemistry and Genetics, Qazvin University of Medical Sciences, Qazvin, IR Iran; Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Clinical Biochemistry and Genetics, Qazvin University of Medical Sciences, Qazvin, IR Iran; Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Nemattollah Gheibi Nemattollah Gheibi Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Fahimeh Torkman Fahimeh Torkman Department of Orgonomy, Hamadan University of Medical Sciences, Hamadan, IR Iran Department of Orgonomy, Hamadan University of Medical Sciences, Hamadan, IR Iran Zohreh Abdolvahabi Zohreh Abdolvahabi Department of Clinical Biochemistry and Genetics, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Clinical Biochemistry and Genetics, Qazvin University of Medical Sciences, Qazvin, IR Iran Farhad Khabbaz Farhad Khabbaz Department of Clinical Biochemistry and Genetics, Qazvin University of Medical Sciences, Qazvin, IR Iran Department of Clinical Biochemistry and Genetics, Qazvin University of Medical Sciences, Qazvin, IR Iran
en 10.17795/bhs-19193 Cleaning From the Inside: Biodegradation of Organophosphate Pesticides by Pseudomonas plecoglossicida Cleaning From the Inside: Biodegradation of Organophosphate Pesticides by <italic>Pseudomonas plecoglossicida</italic> research-article research-article Background

Diazinon is one of the most widely used organophosphrous pesticides in the world may affect animals and human.

Objectives

This study was done to assess whether Pseudomonas plecoglossicida can grow on diazinon as the sole carbon metabolic source in laboratory and agricultural soil.

Materials and Methods

Serial dilutions of pure and commercial diazinon in minimal salt medium were made and 2.5 micro liters of these liquid cultures before and after inoculation with Pseudomonas plecoglossicida were injected to a high performance liquid chromatography column, and subsequently the bacterial growth along with the concentration of diazinon were measured by high performance liquid chromatography (HPLC).

Results

Results of this study indicated that Pseudomonas plecoglossicida can grow not only in Minimal salt Medium but also in the diazinon contaminated soil.

Conclusions

Our findings showed that Pseudomonas plecoglossicida grows in Minimal salt Medium supplemented with serial dilutions of diazinon. Furthermore, this bacterium is capable to biodegrade the residual diazinon in the agricultural soil.

Background

Diazinon is one of the most widely used organophosphrous pesticides in the world may affect animals and human.

Objectives

This study was done to assess whether Pseudomonas plecoglossicida can grow on diazinon as the sole carbon metabolic source in laboratory and agricultural soil.

Materials and Methods

Serial dilutions of pure and commercial diazinon in minimal salt medium were made and 2.5 micro liters of these liquid cultures before and after inoculation with Pseudomonas plecoglossicida were injected to a high performance liquid chromatography column, and subsequently the bacterial growth along with the concentration of diazinon were measured by high performance liquid chromatography (HPLC).

Results

Results of this study indicated that Pseudomonas plecoglossicida can grow not only in Minimal salt Medium but also in the diazinon contaminated soil.

Conclusions

Our findings showed that Pseudomonas plecoglossicida grows in Minimal salt Medium supplemented with serial dilutions of diazinon. Furthermore, this bacterium is capable to biodegrade the residual diazinon in the agricultural soil.

Diazinon;Pseudomonas;Biodegradation Diazinon;Pseudomonas;Biodegradation http://www.Biotech-health.com/index.php?page=article&article_id=19193 Abasalt Borji Abasalt Borji Department of Microbiology, Neyshabur University of Medical Sciences, Neyshabur, IR Iran Department of Microbiology, Neyshabur University of Medical Sciences, Neyshabur, IR Iran Ghazal Naserpour Farivar Ghazal Naserpour Farivar Department of Mining and Oil Engineering, Faculty of Engineering, Imam Khomeini International University, Qazvin, IR Iran Department of Mining and Oil Engineering, Faculty of Engineering, Imam Khomeini International University, Qazvin, IR Iran Pouran Johari Pouran Johari Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Taghi Naserpour Farivar Taghi Naserpour Farivar Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran; Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel: +98-2813324971, Fax: +98-2813324971 Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran; Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel: +98-2813324971, Fax: +98-2813324971 Saeideh Senemari Saeideh Senemari Department of Mining and Oil Engineering, Faculty of Engineering, Imam Khomeini International University, Qazvin, IR Iran Department of Mining and Oil Engineering, Faculty of Engineering, Imam Khomeini International University, Qazvin, IR Iran Gholamrea Karimi Gholamrea Karimi Department of Mining and Oil Engineering, Faculty of Engineering, Imam Khomeini International University, Qazvin, IR Iran Department of Mining and Oil Engineering, Faculty of Engineering, Imam Khomeini International University, Qazvin, IR Iran
en 10.17795/bhs-19194 Sensitivity and Specificity of TaqMan Real Time PCR, PCR, Microscopy and Culture in Diagnosis of Tuberculous Meningitis in a High Incidence of Tuberculosis Province in Southeast of Iran Sensitivity and Specificity of TaqMan Real Time PCR, PCR, Microscopy and Culture in Diagnosis of Tuberculous Meningitis in a High Incidence of Tuberculosis Province in Southeast of Iran research-article research-article Results

Our study showed that the sensitivity and specificity of TaqMan real time PCR was 96% and 95% respectively. These values were 89% and 90, 38% and 100%, 6% and 100% for PCR, culture and microscopy, respectively.

Conclusions

Our study showed that sensitivity of TaqMan real time PCR was higher that PCR, culture and microscopy but specificity of culture and microscopy was more than PCR and even TaqMan real time PCR.

Objectives

We did this study to compare sensitivity and specificity of TaqMan real time PCR, PCR, microscopy and culture in diagnosis of TBM.

Patients and Methods

We had 49 patients with primary diagnosis of TBM during January 2007 and January 2008 in Bou-Ali University Hospital, Southeast of Iran. Combining and using the definite and probable TB as a gold standard, 29 of these patients had a final diagnosis of TBM. The extracted DNA of samples was applied for conventional PCR and TaqMan real time PCR.

Background

The most dangerous form of extra-pulmonary tuberculosis is tuberculous meningitis (TBM). Diagnosis of TBM has special problem due to its paucibacillary. Also, sensitivity and specificity of routine microscopy and culture in the diagnosis of this disease is controversial. So, faster and more accurate laboratory test is required. Polymerase chain reaction (PCR) and real time PCR may be good candidates for this purpose.

Results

Our study showed that the sensitivity and specificity of TaqMan real time PCR was 96% and 95% respectively. These values were 89% and 90, 38% and 100%, 6% and 100% for PCR, culture and microscopy, respectively.

Conclusions

Our study showed that sensitivity of TaqMan real time PCR was higher that PCR, culture and microscopy but specificity of culture and microscopy was more than PCR and even TaqMan real time PCR.

Objectives

We did this study to compare sensitivity and specificity of TaqMan real time PCR, PCR, microscopy and culture in diagnosis of TBM.

Patients and Methods

We had 49 patients with primary diagnosis of TBM during January 2007 and January 2008 in Bou-Ali University Hospital, Southeast of Iran. Combining and using the definite and probable TB as a gold standard, 29 of these patients had a final diagnosis of TBM. The extracted DNA of samples was applied for conventional PCR and TaqMan real time PCR.

Background

The most dangerous form of extra-pulmonary tuberculosis is tuberculous meningitis (TBM). Diagnosis of TBM has special problem due to its paucibacillary. Also, sensitivity and specificity of routine microscopy and culture in the diagnosis of this disease is controversial. So, faster and more accurate laboratory test is required. Polymerase chain reaction (PCR) and real time PCR may be good candidates for this purpose.

Sensitivity;Specificity;Real-Time Polymerase Chain Reaction;Polymerase Chain Reaction Sensitivity;Specificity;Real-Time Polymerase Chain Reaction;Polymerase Chain Reaction http://www.Biotech-health.com/index.php?page=article&article_id=19194 Taghi Naserpour Farivar Taghi Naserpour Farivar Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran; Cell and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel: +98-9128801401, Fax: +98-2813324971 Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran; Cell and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel: +98-9128801401, Fax: +98-2813324971 Pouran Johari Pouran Johari Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Mohammad Hashemi Shahri Mohammad Hashemi Shahri Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, IR Iran Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, IR Iran Mohammad Naderi Mohammad Naderi Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, IR Iran Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, IR Iran Roya Alavi Roya Alavi Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, IR Iran Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, IR Iran Batoul Sharifi-Mood Batoul Sharifi-Mood Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, IR Iran Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, IR Iran
en 10.17795/bhs-19195 Effect of Lentinan in Induction of Apoptosis on Gastric Adenocarcinoma Cells Effect of Lentinan in Induction of Apoptosis on Gastric Adenocarcinoma Cells research-article research-article Background

Gastric cancer is the second most common cause of cancer death worldwide. Lentinan was shown to induce apoptosis in gastric cancer cells and could be used for the treatment of gastric cancer.

Objectives

In this study, we analyzed anticancer effect of lentinan, a fungal β-glucan, on the gastric adenocarcinoma cell line (AGS).

Materials and Methods

We used the DNA ladder and TUNNEL approaches to evaluate the apoptotic effect of lentinan on the AGS cell.

Results

Evaluation of apoptosis by Apoptotic DNA Ladder in lentinan treated and untreated AGS cells by DNA laddering and fragmentation, and TUNEL tests confirmed that application lentinan caused a significant increase in apoptosis in the AGS cell line.

Conclusions

Treatment of human gastric adenocarcinoma cell line with lentinan can offer a possible approach to counteract the human gastric adenocarcinoma cells, thus can be applied in a combination with the routine gastric cancer therapy drugs.

Background

Gastric cancer is the second most common cause of cancer death worldwide. Lentinan was shown to induce apoptosis in gastric cancer cells and could be used for the treatment of gastric cancer.

Objectives

In this study, we analyzed anticancer effect of lentinan, a fungal β-glucan, on the gastric adenocarcinoma cell line (AGS).

Materials and Methods

We used the DNA ladder and TUNNEL approaches to evaluate the apoptotic effect of lentinan on the AGS cell.

Results

Evaluation of apoptosis by Apoptotic DNA Ladder in lentinan treated and untreated AGS cells by DNA laddering and fragmentation, and TUNEL tests confirmed that application lentinan caused a significant increase in apoptosis in the AGS cell line.

Conclusions

Treatment of human gastric adenocarcinoma cell line with lentinan can offer a possible approach to counteract the human gastric adenocarcinoma cells, thus can be applied in a combination with the routine gastric cancer therapy drugs.

Stomach Neoplasms;Apoptosis;Lentinan Stomach Neoplasms;Apoptosis;Lentinan http://www.Biotech-health.com/index.php?page=article&article_id=19195 Reza Najafipour Reza Najafipour Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Taghi Naserpour Farivar Taghi Naserpour Farivar Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran; Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel/Fax: +98-2813324971 Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran; Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel/Fax: +98-2813324971 Pouran Johari Pouran Johari Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran