Biotechnology and Health Sciences Biotechnology and Health Sciences Biotech Health Sci http://www.Biotech-health.com 2383-0271 2383-028X 10.5812/bhs en jalali 2017 6 27 gregorian 2017 6 27 S 1
en 10.5812/bhs-43712 Simultaneous Detection of Pathogenic and Saprophyte Leptospira in Human Plasma by Multiplex Taqman Real Time PCR Simultaneous Detection of Pathogenic and Saprophyte Leptospira in Human Plasma by Multiplex Taqman Real Time PCR research-article research-article Background

Leptospirosis is an infectious disease caused by pathogenic and saprophytic Leptospira species. The clinical and laboratory diagnosis of this infection is complicated. However, timely diagnosis of leptospirosis is essential for treatment of this disease. Conventional laboratory methods are incapable in the early diagnosis of it. Molecular tests such as real time PCR are very efficient when diagnosing it.

Results

Of the 250 samples, 93 (37.2%) were positive for pathogenic and 15 (6%) for non-pathogenic cases. In two samples, pathogenic and non-pathogenic DNA strains were simultaneously positive.

Conclusions

Based on our finds, the real time PCR is a suitable test for the diagnosis of leptospirosis and differentiation between pathogen and saprophyte Leptospira simultaneously.

Methods

250 human plasma samples were obtained from suspected patients. Two pair specific primers and the corresponding probe for detecting pathogenic and saprophytic Leptospira were designed and established in a single tube. The developed tests were run on all DNA extracted from the samples.

Objectives

In this study, we designed and developed a multiplex Taqman real time PCR to simultaneously detect saprophyte and pathologic Leptospira in clinical samples.

Background

Leptospirosis is an infectious disease caused by pathogenic and saprophytic Leptospira species. The clinical and laboratory diagnosis of this infection is complicated. However, timely diagnosis of leptospirosis is essential for treatment of this disease. Conventional laboratory methods are incapable in the early diagnosis of it. Molecular tests such as real time PCR are very efficient when diagnosing it.

Results

Of the 250 samples, 93 (37.2%) were positive for pathogenic and 15 (6%) for non-pathogenic cases. In two samples, pathogenic and non-pathogenic DNA strains were simultaneously positive.

Conclusions

Based on our finds, the real time PCR is a suitable test for the diagnosis of leptospirosis and differentiation between pathogen and saprophyte Leptospira simultaneously.

Methods

250 human plasma samples were obtained from suspected patients. Two pair specific primers and the corresponding probe for detecting pathogenic and saprophytic Leptospira were designed and established in a single tube. The developed tests were run on all DNA extracted from the samples.

Objectives

In this study, we designed and developed a multiplex Taqman real time PCR to simultaneously detect saprophyte and pathologic Leptospira in clinical samples.

Leptospira;Real Time PCR;Saprophyte;Leptospirosis Leptospira;Real Time PCR;Saprophyte;Leptospirosis http://www.Biotech-health.com/index.php?page=article&article_id=43712 Safar Ali Alizadeh Safar Ali Alizadeh Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran Amir Javadi Amir Javadi School of Allied Sciences, Tehran University of Medical Sciences, Tehran, IR Iran School of Allied Sciences, Tehran University of Medical Sciences, Tehran, IR Iran Sajjad Alizadeh Sajjad Alizadeh Tehran University of Medical Sciences, Tehran, IR Iran Tehran University of Medical Sciences, Tehran, IR Iran Reza Najafipour Reza Najafipour Department of Biochemistry, Genetics Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran; Department of Biochemistry, Genetics Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel/Fax: +98-2833324971 Department of Biochemistry, Genetics Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran; Department of Biochemistry, Genetics Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel/Fax: +98-2833324971 Taghi Naserpour Farivar Taghi Naserpour Farivar Department of Microbiology, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran; Department of Microbiology, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel/Fax: +98-2833324971 Department of Microbiology, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran; Department of Microbiology, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran. Tel/Fax: +98-2833324971